In this project, we want to deepen the knowledge of interphase chromatin organization. Human aneuploid carcinoma cell line and human diploid fibroblasts will serve as model systems in these experiments. Four mutually intermingled issues will be pursued: 1. organization of human active replicon 2. dynamics of histone H1 in replicated human chromatin and its role in the organization of replicated chromatin, 3. organization of human replicons in replication factories 4. organization of human interphase chromatin. (en)
1. Popis organizace replikačně aktivního replikonu. 2. Analýza dynamiky histonu H1 v replikovaném chromatinu a jeho role při organizaci replikovaného chromatinu. 3. Popis organizace replikonů uvnitř %22replikačních továren%22. 4. Popis organizace chromatinu v interfázi buněčného cyklu.