About: Characterization of porcine reproductive and respiratory syndrome ( PRRS ) virus strains isolated in the Czech Republic     Goto   Sponge   NotDistinct   Permalink

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  • Budou charakterizovány kmeny viru reprodukčního a respiračního syndromu prasat ( PRRS ) izolované v České republice v r. 1995-1998.Ověřena bude schopnost replikace izolátů na buněčné linii MARC-145 a vliv lyofilizace a dlouhodobého uchovávání lyofilizátů na infekčnos viru .Ke genotypizaci izolátů bude použita metoda RT-PCR a metoda přímé sekvenace PCR produktů.Bude ověřen vliv dlouhodobého pasážování izolátu CAPM V-502 na možné změny v některých úsecích virového genomu a na patogenitu izolátu pro prasata. Budou připraveny, testovány a srovnávány monoklonální protilátky proti viru PRR a jejich využitelnost v diagnostice a při diferenciaci virových kmenů. (cs)
  • Characterisation of the strains of porcine reproductive and respiratory syndrome ( PRRS ) isolated in the Czech Republic in 1995-1998 will include tests of potential to replicate in the cell line MARC-145 and investigations of the effect of freeze -drying and long-term storage of freeze-dried strains on infectiosity. The strains will be typified using RT-PCR and direct sequencing of PCR products. Effects of long-term passaging of the strain CAPM V-502 on selected regions of viral genome and pathogenicity for swine will be tested. Monoclonal antibodies to PRRS virus will be prepared and tested for their application in diagnostics and differentiation of virus strains. (en)
Title
  • Characterization of porcine reproductive and respiratory syndrome ( PRRS ) virus strains isolated in the Czech Republic (en)
  • Charakteristika kmenů viru reprodukčního a respiračního syndromu prasat(PRRS) izolovaných v ČR. (cs)
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  • virus PRRS, RT-PCR, (en)
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