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  • V předloženém projektu navrhujeme komplexně vyšetřit nádorový supresor p53 v nádorové tkáni pacientů s MCL a DLBCL s cílem zjistit, zda je aberace genu p53 využitelným prognostickým markerem tohoto onemocnění. K detekci aberací p53 bude využita funkční analýza separovaných alel v kvasinkách, sekvenování DNA, analýza hladiny proteinu p53 imunohiostochemicky a imunoblotingem a analýza delece lokusu 17p13 pomocí FISH. (cs)
  • The aim of this project is complex analysis of tumor suppressor p53 in tumor tissue of patients with diffuse large B-cell lymphoma and mantle cell lymphoma to verify whether p53 abberation is useful prognostic marker of the diseases. For detection of p53 abberations, we will employ funtional analysis of separated alleles in yeast (FASAY), DNA sequencing, analysis of the p53 protein level by immunohistochemistry and immunoblotting, and analysis of the locus 17p13 by FISH. (en)
Title
  • Komplexní analýza nádorového supresoru p53 u nehodgkinských lymfomů (cs)
  • Complex analysis of p53 tumor suppressor in non-Hodgkin´s lymphomas (en)
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  • non-Hodgkin´s lymphoma; diffuse large B-cell lymphoma; mantle cell lymphoma; tumor suppressor p53; FASAY; FISH; immunohistochemistry; tumor suppressor ATM (en)
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