About: Experimentally modified gene expression of BRCA1 and BRCA2 variants in primary mammary cells in vitro: contribution to breast cancer development and sensitivity to diverse options of cancer therapy in BRCA1/2 genes mutation carriers

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About: Experimentally modified gene expression of BRCA1 and BRCA2 variants in primary mammary cells in vitro: contribution to breast cancer development and sensitivity to diverse options of cancer therapy in BRCA1/2 genes mutation carriers Goto Sponge NotDistinct Permalink

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rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
Description	"The aim of the project is characterization of cellular changes produced by expression of mutant BRCA1 and BRCA2 genes in the in vitro model of mammary cells cultivated from breast glandular structures of BRCA1/2 mutation carriers. Following fine-needle biopsy, the cells will be cultivated using ""feeder layer technique"". BRCA1/2 wt allele expression will be silenced using RNA interference. BRCA1/2-dependent gene expression will be studied using cDNA arrays. Selected targets of altered BRCA1/2 signalization will be quantified using qRT-PCR and western blotting; mislocalization of gene products will be fractionated using centrifugation. The effect of anticancer therapy will be studied in primary mammary cells expressing wt, or mutant, or both alleles of the BRCA1/2 specified gene alterations." (en) "Cílem projektu je funkční charakterizace exprese mutantních forem genů BRCA1 a BRCA2 v in vitro modelu primárních buněk kultivovaných ze žlázové tkáně prsu nosiček mutací v genech BRCA1 nebo BRCA2. Po odběru punkční biopsií budou buňky kultivovány s využitím ""feeder-layer"" techniky. V buňkách in vitro bude exprese wt alely inhibována pomocí RNA interference. Studována bude BRCA1/2-dependentní exprese genů, resp. její alterace, pomocí cDNA array. Charakterizace cílů defektní signalizace BRCA1/2 bude kvantifikována pomocí qRT-PCR a western blottingu; defektní lokalizace genových produktů bude studována pomocí separační centrifugace. V primárních buňkách bude studován účinek protinádorových léčiv v závislosti na diferencované expresi mutantních resp. wt forem genů BRCA1/2." (cs)
Title	Experimentally modified gene expression of BRCA1 and BRCA2 variants in primary mammary cells in vitro: contribution to breast cancer development and sensitivity to diverse options of cancer therapy in BRCA1/2 genes mutation carriers (en) Experimentálně modifikovaná exprese sekvenčních variant genů BRCA1 a BRCA2 v primárních mamárních buňkách in vitro: vliv na vznik karcinomu prsu a jeho ovlivnění různými modalitami protinádorové léčby u nosičů hereditárních mutací (cs)
http://linked.open...vai/cislo-smlouvy	http://linked.opendata.cz/resource/domain/vavai/cep/smlouva/NR8345-4/2005
http://linked.open...avai/druh-souteze	http://linked.opendata.cz/resource/domain/vavai/cep/druh-souteze/VS
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http://linked.open...n/vavai/kategorie	http://linked.opendata.cz/resource/domain/vavai/cep/kategorie/21
http://linked.open...vai/klicova-slova	breast cancer; mutation; BRCA1; RNA interference; primary culture; heredity; BRCA2; cytostatics (en)
http://linked.open...avai/konec-reseni	2008-12-31 (xsd:date)
http://linked.open...nujicich-prijemcu	0 (xsd:integer)
http://linked.open...avai/poskytovatel	http://linked.opendata.cz/resource/domain/vavai/cep/poskytovatel/MZ0
http://linked.open...avai/start-reseni	2005-01-01 (xsd:date)
http://linked.open...ai/statni-podpora	4934 (xsd:integer)
http://linked.open...vavai/typProjektu	http://linked.opendata.cz/resource/domain/vavai/cep/typ/P
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http://linked.open...ku-zverejnovanych	4 (xsd:integer)
is http://linked.open...ain/vavai/projekt of	http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2005/NR8345/ico-68378050 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2005/NR8345/orjk-11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2006/NR8345/ico-68378050 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2006/NR8345/orjk-11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2007/NR8345/ico-68378050 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2007/NR8345/orjk-11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2008/NR8345/ico-68378050 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2008/NR8345/orjk-11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2009/NR8345/ico-68378050 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/2009/NR8345/orjk-11110

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