About: Inappropriate Activation of the Transforming Growth Factor-betal in the SclerodermaI Tissue:: Transglutaminase Factor XIIIa Strikes the Cord?     Goto   Sponge   NotDistinct   Permalink

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Description
  • V kožních biopsiích,buňkách periferní krve a fibroblastech pacientů s lokalizovanou a systémovou sklerodermií bude hodnocena přítomnost Faktoru XIIIa,TGF-beta1,kolagenuI,Borrelia burgdorfeli na úrovni proteinu (imunohistochemické barvení,Western blottingDále bude měřena biologická aktivita TGF-beta1 a Faktoru XIIIa in vitro za použití buněčné linie citlivé na přítomnost TGF-beta1 a substrátu modifikovaného působením Faktoru XIIIa. (cs)
  • Skin biopsies,blood leukocytes and cultured fibroblasts of the patients with the local and systemic scleroderma will be investigated for Factor XIIIa,TGF-beta1,collagen I. Borrelia burgdorferi protein (immunostaining,Western blotting),mRNA (RT-PCR,North Borrelia burgdorferi protein (immunostaining,Western blotting),mRNA (RT-PCR,Northern blotting) and DNA (PCR). Biological activity of TGF-beta1 and Factor XIIIa will be measured in vitro using TGF-beta1-sensitive cell line and Factor XIIIa-sensitive substrate,respectively. (en)
Title
  • "Abnormální aktivace transformačního růstového faktoru-betal ve sklerodermické tkáni : sólo pro transglutaminázu ""faktor XIIIa""?" (cs)
  • Inappropriate Activation of the Transforming Growth Factor-betal in the SclerodermaI Tissue:: Transglutaminase Factor XIIIa Strikes the Cord? (en)
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