Hlavním cílem projektu bude snaha o celkovou charakterizaci nové rodiny WrbA proteinů na molekulární úrovni a výzkum biologické funkce těchto proteinů. (cs)
The objective of the proposed work is to understand the relationship between structure and function for the E. coli protein WrbA, the founding member of a widespread and highly conserved family of proteins. Although WrbA is related to the well-studied bacterial FMN-dependent flavodoxin electron carriers and eukaryotic FAD-dependent oxidoreductases, it presents significant distinctions from both, and has a completely unknown physiological role. The very wide phylogenetic distribution of WrbA homologs suggests an important metabolic role that may reveal unexpected evolutionary and ecological relationships among distant organisms. New hypotheses that follow from our previous work on WrbA structures will be evaluated in a comparative structure/function framework with its better-understood relatives. The aims are related to our long-term interests in the molecular origins of intramolecular structural organization, stability, and dynamics, and of intermolecular affinity, specificity, and cooperativity. The (en)