About: Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Easy, efficient and low demanding separation of mRNA from biological material is needed to study gene expression and to use in chip technologies. It is common knowledge that each mRNA molecule contains sequence of 25 adenines. This feature can be used for binding mRNA on the surface of the particles coated by thymine chains. The present work reports on suggesting and optimizing of mRNA separation and detection from biological material via paramagnetic microparticles coupled with electrochemical detection. Primarily we optimized cyclic and square wave voltammetric conditions to detect poly(A),which was used as standard to mimic behaviour of mRNA. Under the optimized square wave voltammetric conditions (frequency 280 Hz, accumulation time 200 s, supporting electrolyte and its temperature: acetate buffer 4.6 and 35 degrees C) we estimated detection limit down to 1 ng of poly(A) per ml. To enhance effectiveness and repeatability of isolation of nucleic acid automated approach for rinsing and hybridizing w Easy, efficient and low demanding separation of mRNA from biological material is needed to study gene expression and to use in chip technologies. It is common knowledge that each mRNA molecule contains sequence of 25 adenines. This feature can be used for binding mRNA on the surface of the particles coated by thymine chains. The present work reports on suggesting and optimizing of mRNA separation and detection from biological material via paramagnetic microparticles coupled with electrochemical detection. Primarily we optimized cyclic and square wave voltammetric conditions to detect poly(A),which was used as standard to mimic behaviour of mRNA. Under the optimized square wave voltammetric conditions (frequency 280 Hz, accumulation time 200 s, supporting electrolyte and its temperature: acetate buffer 4.6 and 35 degrees C) we estimated detection limit down to 1 ng of poly(A) per ml. To enhance effectiveness and repeatability of isolation of nucleic acid automated approach for rinsing and hybridizing w (en)
Title	Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection (en)
skos:prefLabel	Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection (en)
skos:notation	RIV/00216305:26220/09:PU84346!RIV10-GA0-26220___
http://linked.open...avai/riv/aktivita	P V
http://linked.open...avai/riv/aktivity	P(GA102/08/1546), P(KAN208130801), V
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hubálek, Jaromír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta elektrotechniky a komunikačních technologií
http://linked.open...dnocenehoVysledku	304455
http://linked.open...ai/riv/idVysledku	RIV/00216305:26220/09:PU84346
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	mRNA, Isolation, Magnetic particle and bead, Cyclic voltammetry, Square wave voltammetry, Brain tissue, Maize plants, Cadmium (en)
http://linked.open.../riv/klicoveSlovo	Isolation Brain tissue Magnetic particle and bead Maize plants Cadmium Cyclic voltammetry mRNA Square wave voltammetry
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[074E7B739C55]
http://linked.open...i/riv/nazevZdroje	Talanta
http://linked.open...in/vavai/riv/obor	JB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	New design and exploiting nanobiosensors and nanosensors to target medicine (NANOSEMED) Miniaturized intelligent systems and nanostructured elektrodes for chemical, biological, and pharmaceutical applications (NANIMEL)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Havel, Ladislav Hubálek, Jaromír Horna, Aleš Húska, Dalibor Kizek, René Trnková, Libuše Vajtr, David
issn	0039-9140
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	26220
is http://linked.open...avai/riv/vysledek of	Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection

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