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| rdf:type
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| Description
| - The diagnostic use of in vitro molecular assays can be limited by the lack of guidelines for collection, handling, stabilization and storage of patient specimens. One of the major goals of the EC funded project SPIDIA (www.spidia.eu) is to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for molecular testing which requires intracellular RNA analytes. To this end, a survey and a pan-European external quality assessment (EQA) were implemented. This report is the summary of the results of that trial. With the European Federation of Laboratory Medicine (EFLM) support, 124 applications for participation in the trial were received from 27 different European countries, and 102 laboratories actually participated in the trial. Each participating laboratory described their respective laboratory policies and practices as well as blood collection tubes typically used in performing this type of testing. The participating laboratories received two identical blood specimens: in an EDTA tubes (unstabilized blood; n = 67) or in tubes designed specifically for the stabilization of intracellular RNA in blood (PAXgene Blood RNA tubes; n = 35). Laboratories were requested to perform RNA extraction according to the laboratory’s own procedure as soon as possible upon receipt of the tubes for one tube and 24 h after the first extraction for the second tube. Participants (n = 93) returned the two extracted RNAs to SPIDIA facility for analysis, and provided details about the reagents and protocols they used for the extraction.
- The diagnostic use of in vitro molecular assays can be limited by the lack of guidelines for collection, handling, stabilization and storage of patient specimens. One of the major goals of the EC funded project SPIDIA (www.spidia.eu) is to develop evidence-based quality guidelines for the pre-analytical phase of blood samples used for molecular testing which requires intracellular RNA analytes. To this end, a survey and a pan-European external quality assessment (EQA) were implemented. This report is the summary of the results of that trial. With the European Federation of Laboratory Medicine (EFLM) support, 124 applications for participation in the trial were received from 27 different European countries, and 102 laboratories actually participated in the trial. Each participating laboratory described their respective laboratory policies and practices as well as blood collection tubes typically used in performing this type of testing. The participating laboratories received two identical blood specimens: in an EDTA tubes (unstabilized blood; n = 67) or in tubes designed specifically for the stabilization of intracellular RNA in blood (PAXgene Blood RNA tubes; n = 35). Laboratories were requested to perform RNA extraction according to the laboratory’s own procedure as soon as possible upon receipt of the tubes for one tube and 24 h after the first extraction for the second tube. Participants (n = 93) returned the two extracted RNAs to SPIDIA facility for analysis, and provided details about the reagents and protocols they used for the extraction. (en)
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| Title
| - SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses
- SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses (en)
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| skos:prefLabel
| - SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses
- SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses (en)
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| skos:notation
| - RIV/86652036:_____/13:00392025!RIV14-AV0-86652036
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/86652036:_____/13:00392025
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Pre-analytical phase; RNA quality; Blood samples (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Kubista, Mikael
- Tichopád, Aleš
- Gelmini, S.
- Guenther, K.
- Malentacchi, F.
- Pazzagli, M.
- Pizzamiglio, S.
- Verderio, P.
- Wyrich, R.
- Ciniselli, C. M.
- Hartmann, C.
- Simi, L.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1016/j.ymeth.2012.10.007
|
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