About: 3 days Hands-on qPCR     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification. Day 3 - Sample Preparation (Extraction of RNA and DNA). Principles of RT. Priming methods for RT. RT optimization. The MIQE guidelines. Group discussion, bring your own questions.
  • Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification. Day 3 - Sample Preparation (Extraction of RNA and DNA). Principles of RT. Priming methods for RT. RT optimization. The MIQE guidelines. Group discussion, bring your own questions. (en)
Title
  • 3 days Hands-on qPCR
  • 3 days Hands-on qPCR (en)
skos:prefLabel
  • 3 days Hands-on qPCR
  • 3 days Hands-on qPCR (en)
skos:notation
  • RIV/86652036:_____/12:00389223!RIV13-AV0-86652036
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50520701)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 183383
http://linked.open...ai/riv/idVysledku
  • RIV/86652036:_____/12:00389223
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • qPCR; biostatistic; reverese transcription (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [5A0B1698830B]
http://linked.open...v/mistoKonaniAkce
  • Praha, BTÚ
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...UplatneniVysledku
http://linked.open...iv/statKonaniAkce
http://linked.open...iv/tvurceVysledku
  • Kubista, Mikael
  • Korenková, Vlasta
  • Langerová, Lucie
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/ukonceniAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software