About: Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

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About: Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizing sperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage. Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizing sperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage. (en)
Title	Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization (en)
skos:prefLabel	Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization (en)
skos:notation	RIV/86652036:_____/11:00371924!RIV12-AV0-86652036
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA500520809), Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kubista, Mikael Rusňáková, Vendula
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	222675
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/11:00371924
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Single-cell gene expression; Glycidamide; Acrylamide (en)
http://linked.open.../riv/klicoveSlovo	Acrylamide Glycidamide Single-cell gene expression
http://linked.open...odStatuVydavatele	NO - Norské království
http://linked.open...ontrolniKodProRIV	[9F6A48F0DFD3]
http://linked.open...i/riv/nazevZdroje	Reproductive Toxicology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Mechanisms of embryonic stem cells early differentiation
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Kubista, Mikael Brevik, A. Brunborg, G. Duale, N. Lindeman, B. Olsen, A.-K. Rusňáková, Vendula Slagsvold, H. H. Storeng, R.
http://linked.open...ain/vavai/riv/wos	000297716900010
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR
issn	0890-6238
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.reprotox.2011.09.005

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