About: Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein

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About: Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Porcine beta-microseminoprotein (MSP) has been described as a protein characteristic of the prostate; however, we proved its mRNA transcripts in all reproductive organs. In tissue extracts, specific antibody antiMSP recognized a 12-kDa protein band, which was identified by mass spectrometry as MSP, and immunofluorescence displayed the MSP occurrence in epithelial tissue. MSP was localized on epididymal sperm in the acrosomal region and in addition to that on the flagellum of ejaculated sperm. The MSP absence on the surface of capacitated sperm together with the antibody detection of MSP in the sperm acidic extract after in vitro capacitation indicated its acrosomal origin. The MSP sperm localization was confirmed by electron microscopy. The secretion of porcine MSP by different reproductive organs might indicate its multiple roles in the reproductive process, and for the first time in mammalian species, the MSP localization has been shown at different physiological stages of sperm. Porcine beta-microseminoprotein (MSP) has been described as a protein characteristic of the prostate; however, we proved its mRNA transcripts in all reproductive organs. In tissue extracts, specific antibody antiMSP recognized a 12-kDa protein band, which was identified by mass spectrometry as MSP, and immunofluorescence displayed the MSP occurrence in epithelial tissue. MSP was localized on epididymal sperm in the acrosomal region and in addition to that on the flagellum of ejaculated sperm. The MSP absence on the surface of capacitated sperm together with the antibody detection of MSP in the sperm acidic extract after in vitro capacitation indicated its acrosomal origin. The MSP sperm localization was confirmed by electron microscopy. The secretion of porcine MSP by different reproductive organs might indicate its multiple roles in the reproductive process, and for the first time in mammalian species, the MSP localization has been shown at different physiological stages of sperm. (en)
Title	Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein (en)
skos:prefLabel	Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein (en)
skos:notation	RIV/86652036:_____/11:00367617!RIV12-MZ0-86652036
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M06011), P(GA303/09/1285), P(GD523/08/H064), P(LC07017), P(LC545), P(NS10009), S, Z(AV0Z50200510), Z(AV0Z50520514), Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Jonáková, Věra Cozlová, Nina Maňásková-Postlerová, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	226622
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/11:00367617
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	porcine beta-microseminoprotein; reproductive organs; spermatozoa; immunofluorescence; sperm acrosome; boar (en)
http://linked.open.../riv/klicoveSlovo	porcine beta-microseminoprotein reproductive organs sperm acrosome boar immunofluorescence spermatozoa
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[B5BB441B1CEE]
http://linked.open...i/riv/nazevZdroje	Cell and Tissue Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Expression of genes and proteins playing a role in the human reproduction - damage biomarkers The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment. Functional Organisation of the Cell Oncoproteomics Centre Study of the sperm membrane proteins with zona pellucida binding activity Biotechnologies of mammalian gametes
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	344
http://linked.open...iv/tvurceVysledku	Davidová, Nina Hozák, Pavel Jonáková, Věra Maňásková-Postlerová, Pavla Šulc, Miroslav Philimonenko, Anatoly
http://linked.open...ain/vavai/riv/wos	000290167600016
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů Establishing of the Biotechnology Institute ASCR
issn	0302-766X
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00441-011-1149-y

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