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  • The MIQE guidelines has rapidly become standard reporting procedure when writing up and presenting real-time quantitative pCR (qPCR) data. Accoeding to the originál requirement of MIQE full disclosure of primer sequence information is requested. This requirement is not possible to meet when using commersial primers from some leading vendors, since they disclose to clients only limited amount of informatik about the primers they supply. Recently they increased the information provided to clients to make experiments easier to design and easier to reproduce. In this letter the MIQE guidelines are amended to allow also these primers to be used in MIQE complient reports and publications.
  • The MIQE guidelines has rapidly become standard reporting procedure when writing up and presenting real-time quantitative pCR (qPCR) data. Accoeding to the originál requirement of MIQE full disclosure of primer sequence information is requested. This requirement is not possible to meet when using commersial primers from some leading vendors, since they disclose to clients only limited amount of informatik about the primers they supply. Recently they increased the information provided to clients to make experiments easier to design and easier to reproduce. In this letter the MIQE guidelines are amended to allow also these primers to be used in MIQE complient reports and publications. (en)
Title
  • Primer Sequence Disclosure: A Clarification of the MIQE Guidelines
  • Primer Sequence Disclosure: A Clarification of the MIQE Guidelines (en)
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  • Primer Sequence Disclosure: A Clarification of the MIQE Guidelines
  • Primer Sequence Disclosure: A Clarification of the MIQE Guidelines (en)
skos:notation
  • RIV/86652036:_____/11:00364331!RIV12-AV0-86652036
http://linked.open...avai/riv/aktivita
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  • Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
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  • 223404
http://linked.open...ai/riv/idVysledku
  • RIV/86652036:_____/11:00364331
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • real-time PCR; MIQE; quality (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [0BC8ABD323B4]
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  • Clinical Chemistry
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http://linked.open...v/svazekPeriodika
  • 57
http://linked.open...iv/tvurceVysledku
  • Kubista, Mikael
  • Müller, R.
  • Benes, V.
  • Bustin, S. A.
  • Garson, J. A
  • Hellemans, J.
  • Huggett, J.
  • Nolan, T.
  • Pfaffl, M. W.
  • Shipley, G. L.
  • Vandesompele, J.
  • Wittwer, C. T.
http://linked.open...ain/vavai/riv/wos
  • 000291028600026
http://linked.open...n/vavai/riv/zamer
issn
  • 0009-9147
number of pages
http://bibframe.org/vocab/doi
  • 10.1373/clinchem.2011.162958
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