About: Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms

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About: Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recognition and the binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. Despite longstanding research efforts to identify the sperm proteins that recognize ZP receptors, their precise determination still remains questionable in various mammalian species. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by agents as follows: Triton X-100, Triton X-114, acetic acid, SDS, n-octyl-β-D-glucopyranosid, extraction by freezing-thawing and characterized by 1D, 2D electrophoreses glycoprotein staining and substrate zymographic methods. Results have shown the quantitative and qualitative differences in 1D, 2D protein and glycoprotein profiles depending upon the isolation protocol. Differences were observed between protein profiles of ejaculated and capacitated sperms as well. Zymograms supported the prediction that using various isolation protocols gives various profiles of enzymatically active molecules. Recognition and the binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. Despite longstanding research efforts to identify the sperm proteins that recognize ZP receptors, their precise determination still remains questionable in various mammalian species. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by agents as follows: Triton X-100, Triton X-114, acetic acid, SDS, n-octyl-β-D-glucopyranosid, extraction by freezing-thawing and characterized by 1D, 2D electrophoreses glycoprotein staining and substrate zymographic methods. Results have shown the quantitative and qualitative differences in 1D, 2D protein and glycoprotein profiles depending upon the isolation protocol. Differences were observed between protein profiles of ejaculated and capacitated sperms as well. Zymograms supported the prediction that using various isolation protocols gives various profiles of enzymatically active molecules. (en)
Title	Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms (en)
skos:prefLabel	Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms Electrophoretic and zymographic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms (en)
skos:notation	RIV/86652036:_____/11:00360805!RIV12-MZ0-86652036
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M06011), P(GA303/09/1285), P(NS10009), S, Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Jonáková, Věra Maňásková-Postlerová, Pavla Zigo, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	197140
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/11:00360805
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Extraction methods; Sperm proteins; Substrate zymography (en)
http://linked.open.../riv/klicoveSlovo	Extraction methods Sperm proteins Substrate zymography
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[E70CA60F59CF]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Expression of genes and proteins playing a role in the human reproduction - damage biomarkers The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment. Study of the sperm membrane proteins with zona pellucida binding activity
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Jonáková, Věra Maňásková-Postlerová, Pavla Zigo, Michal
http://linked.open...ain/vavai/riv/wos	000291555900007
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR
issn	0173-0835
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/elps.201000558

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