About: Biochemical and binding characteristics of boar epididymal fluid proteins

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Proteins and glycoproteins of boar epididymal fluid may be adsorbed on the sperm surface and participate in the sperm maturation, in sperm capacitation, gamete recognition, binding and fusion. Boar epididymal fluid proteins were separated by size exclusion chromatography and by RP-HPLC. The fractions were characterized by SDS-PAGE and proteins were transfered to nitrocellulose membranes and tested for the interaction with biotin-labeled ligands: glycoproteins of zona pellucida (ZP), hyaluronic acid and heparin. Changes in the interaction of epididymal sperm were studied by ELBA. The affinity of epididymal proteins (12-17 kDa, 23 kDa) to heparin and hyaluronic acid suggests their binding ability to oviductal proteoglycans. Epididymal proteins of 12-18 kDa interacted with ZP glycoproteins. We identified Crisp3-like protein. We showed the ability of epididymal fluid fractions to change the interaction of the epididymal sperm surface with ZP glycoproteins, hyaluronic acid and heparin. Proteins and glycoproteins of boar epididymal fluid may be adsorbed on the sperm surface and participate in the sperm maturation, in sperm capacitation, gamete recognition, binding and fusion. Boar epididymal fluid proteins were separated by size exclusion chromatography and by RP-HPLC. The fractions were characterized by SDS-PAGE and proteins were transfered to nitrocellulose membranes and tested for the interaction with biotin-labeled ligands: glycoproteins of zona pellucida (ZP), hyaluronic acid and heparin. Changes in the interaction of epididymal sperm were studied by ELBA. The affinity of epididymal proteins (12-17 kDa, 23 kDa) to heparin and hyaluronic acid suggests their binding ability to oviductal proteoglycans. Epididymal proteins of 12-18 kDa interacted with ZP glycoproteins. We identified Crisp3-like protein. We showed the ability of epididymal fluid fractions to change the interaction of the epididymal sperm surface with ZP glycoproteins, hyaluronic acid and heparin. (en)
Title	Biochemical and binding characteristics of boar epididymal fluid proteins Biochemical and binding characteristics of boar epididymal fluid proteins (en)
skos:prefLabel	Biochemical and binding characteristics of boar epididymal fluid proteins Biochemical and binding characteristics of boar epididymal fluid proteins (en)
skos:notation	RIV/86652036:_____/11:00353966!RIV12-AV0-86652036
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M06011), P(GA303/09/1285), P(GD523/08/H064), S, Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Jonáková, Věra Cozlová, Nina Maňásková-Postlerová, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	188245
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/11:00353966
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	epididymal proteins; RP HPLC; biotin-labeled ligands (en)
http://linked.open.../riv/klicoveSlovo	RP HPLC biotin-labeled ligands epididymal proteins
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[B97A770C2FE9]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment. Study of the sperm membrane proteins with zona pellucida binding activity Biotechnologies of mammalian gametes
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	879
http://linked.open...iv/tvurceVysledku	Davidová, Nina Jonáková, Věra Maňásková-Postlerová, Pavla
http://linked.open...ain/vavai/riv/wos	000286539900015
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR
issn	1570-0232
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.jchromb.2010.11.023

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