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| rdf:type
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| Description
| - Protein ubiquitination is a stable, covalent post-translational modification that alters protein activity and/or targets proteins for proteolysis by the 26S proteasome. The E1-type ubiquitin-activating enzyme (UBA1) is responsible for ubiquitin activation, the initial step of ubiquitin–protein ligation. Proteasomal proteolysis of ubiquitinated spermatozoa and oocyte proteins occurs during mammalian fertilization, particularly at the site of sperm acrosome contact with oocyte zona pellucida. However, it is not clear whether the substrates are solely proteins ubiquitinated during gametogenesis or if de novo ubiquitination also occurs during fertilization supported by ubiquitin-activating and -conjugating enzymes present in the sperm acrosome. Along this line of inquiry, UBA1 was detected in boar extracts by WB. Immunofluorescence revealed accumulation of UBA1 in the nuclei of spermatogonia, spermatocytes and spermatids, and in the acrosomal caps of round and elongating spermatids. Thiol ester assays utilizing biotinylated ubiquitin and isolated sperm acrosomes confirmed the enzymatic activity of the resident UBA1. A specific UBA1 inhibitor, PYR-41, altered the remodelling of OAM during sperm capacitation, monitored using flow cytometry of FITC-PNA. The spermatozoa capacitated in the presence of PYR-41, showed significantly reduced fertilization rates during IVF. Similarly, the fertilization rate was lowered by the addition of PYR-41 directly into fertilization medium during IVF. In WB, high Mr bands, suggestive of protein ubiquitination, were detected in non-capacitated spermatozoa by antibodies against ubiquitin; WB with anti-phosphotyrosine Abs and Abs against acrosomal proteins SPINK2 and AQN1 revealed that the capacitation-induced modification of those proteins was altered by PYR-41. In summary, it appears that de novo protein ubiquitination involving UBA1 contributes to sperm capacitation and acrosomal function during fertilization.
- Protein ubiquitination is a stable, covalent post-translational modification that alters protein activity and/or targets proteins for proteolysis by the 26S proteasome. The E1-type ubiquitin-activating enzyme (UBA1) is responsible for ubiquitin activation, the initial step of ubiquitin–protein ligation. Proteasomal proteolysis of ubiquitinated spermatozoa and oocyte proteins occurs during mammalian fertilization, particularly at the site of sperm acrosome contact with oocyte zona pellucida. However, it is not clear whether the substrates are solely proteins ubiquitinated during gametogenesis or if de novo ubiquitination also occurs during fertilization supported by ubiquitin-activating and -conjugating enzymes present in the sperm acrosome. Along this line of inquiry, UBA1 was detected in boar extracts by WB. Immunofluorescence revealed accumulation of UBA1 in the nuclei of spermatogonia, spermatocytes and spermatids, and in the acrosomal caps of round and elongating spermatids. Thiol ester assays utilizing biotinylated ubiquitin and isolated sperm acrosomes confirmed the enzymatic activity of the resident UBA1. A specific UBA1 inhibitor, PYR-41, altered the remodelling of OAM during sperm capacitation, monitored using flow cytometry of FITC-PNA. The spermatozoa capacitated in the presence of PYR-41, showed significantly reduced fertilization rates during IVF. Similarly, the fertilization rate was lowered by the addition of PYR-41 directly into fertilization medium during IVF. In WB, high Mr bands, suggestive of protein ubiquitination, were detected in non-capacitated spermatozoa by antibodies against ubiquitin; WB with anti-phosphotyrosine Abs and Abs against acrosomal proteins SPINK2 and AQN1 revealed that the capacitation-induced modification of those proteins was altered by PYR-41. In summary, it appears that de novo protein ubiquitination involving UBA1 contributes to sperm capacitation and acrosomal function during fertilization. (en)
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| Title
| - The Activity of Ubiquitin Activating Enzyme UBA1 Is Required for Sperm Capacitation, Acrosornal Exocytosis, and Sperm-Egg Coat Penetration During Porcine Fertilization
- The Activity of Ubiquitin Activating Enzyme UBA1 Is Required for Sperm Capacitation, Acrosornal Exocytosis, and Sperm-Egg Coat Penetration During Porcine Fertilization (en)
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| skos:prefLabel
| - The Activity of Ubiquitin Activating Enzyme UBA1 Is Required for Sperm Capacitation, Acrosornal Exocytosis, and Sperm-Egg Coat Penetration During Porcine Fertilization
- The Activity of Ubiquitin Activating Enzyme UBA1 Is Required for Sperm Capacitation, Acrosornal Exocytosis, and Sperm-Egg Coat Penetration During Porcine Fertilization (en)
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| skos:notation
| - RIV/86652036:_____/10:00372755!RIV12-AV0-86652036
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/86652036:_____/10:00372755
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - ubiquitin; capacitation; fertilization (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Jonáková, Věra
- Sutovsky, M.
- Sutovsky, P.
- Manandhar, G.
- Odhiambo, J. F.
- Zimmermann, S. W.
- Park, C. S.
- Yi, Y. N.
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| http://linked.open...n/vavai/riv/zamer
| |
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