About: Quality control for quantitative PCR based on amplification compatibility test

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Quantitative qPCR is method for the accurate quantification of nucleic acids. Yet it may generate erroneous results if the amplification process is obscured by inhibition or generation of aberrant side-product. Herein we present a statistical approach based on multivariate analysis of the amplification response data generated in qPCR. The amplification trajectory in its most dynamic phase is fitted with a suitable model. Two parameters of this model, related to amplification efficiency, are then used for calculation of the Z-score statistics. Each studied sample is compared to a predefined reference set of reactions. A probabilistic decision for each individual Z-score is then used to identify the majority of inhibited reactions in our experiments. We demonstrate improved identification performance using the multivariate approach Finally we stress that the performance of the amplification kompatibility test as a quality control procedure depends on the quality of the reference set Quantitative qPCR is method for the accurate quantification of nucleic acids. Yet it may generate erroneous results if the amplification process is obscured by inhibition or generation of aberrant side-product. Herein we present a statistical approach based on multivariate analysis of the amplification response data generated in qPCR. The amplification trajectory in its most dynamic phase is fitted with a suitable model. Two parameters of this model, related to amplification efficiency, are then used for calculation of the Z-score statistics. Each studied sample is compared to a predefined reference set of reactions. A probabilistic decision for each individual Z-score is then used to identify the majority of inhibited reactions in our experiments. We demonstrate improved identification performance using the multivariate approach Finally we stress that the performance of the amplification kompatibility test as a quality control procedure depends on the quality of the reference set (en)
Title	Quality control for quantitative PCR based on amplification compatibility test Quality control for quantitative PCR based on amplification compatibility test (en)
skos:prefLabel	Quality control for quantitative PCR based on amplification compatibility test Quality control for quantitative PCR based on amplification compatibility test (en)
skos:notation	RIV/86652036:_____/10:00351162!RIV11-AV0-86652036
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA500520809), P(IAA500970904), Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kubista, Mikael Pecen, Ladislav Tichopád, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	283584
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/10:00351162
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Quantitative PCR; Quality control; Amplification efficiency (en)
http://linked.open.../riv/klicoveSlovo	Quality control Amplification efficiency Quantitative PCR
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[273212DA5C65]
http://linked.open...i/riv/nazevZdroje	Methods
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Mechanisms of embryonic stem cells early differentiation MicroRNA expression during early development of Xenopus laevis and mRNA degradation
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Kubista, Mikael Pecen, Ladislav Pfaffl, M. W. Tichopád, Aleš Bar, T. Kitchen, R. R.
http://linked.open...ain/vavai/riv/wos	000276262100015
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR
issn	1046-2023
number of pages	5 (xsd:int)

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