About: Study of acrosin inhibitor along the boar epididymis

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sperm maturation in the epididymis represents a key step in the reproduction process. Sperm plasma membrane is exposed to epididymal fluid representing the natural environment essential for their post-testicular maturation. Changes in the sperm membrane proteins are influenced by proteolytic and glycosidic enzymes present in the fluid. We monitored acrosin inhibitor (AI) in epididymal fluid and spermatozoa along the organ. Boar epididymal fluid with spermatozoa was isolated according to its spatial restriction into 9 fractions. The AI was detected in sperm extracts and fluids using specific polyclonal antibody. Expression of AI gene was investigated by PCR method. Additionally, immunofluorescence technique (IMF) was used for localization in the tissue. We registered the increasing signal of AI from caput to cauda epididymis. We confirmed that using gene expression method. Using specific antibody, AI was localized in the secretory tissues of caput, corpus and cauda epididymis by IMF. Sperm maturation in the epididymis represents a key step in the reproduction process. Sperm plasma membrane is exposed to epididymal fluid representing the natural environment essential for their post-testicular maturation. Changes in the sperm membrane proteins are influenced by proteolytic and glycosidic enzymes present in the fluid. We monitored acrosin inhibitor (AI) in epididymal fluid and spermatozoa along the organ. Boar epididymal fluid with spermatozoa was isolated according to its spatial restriction into 9 fractions. The AI was detected in sperm extracts and fluids using specific polyclonal antibody. Expression of AI gene was investigated by PCR method. Additionally, immunofluorescence technique (IMF) was used for localization in the tissue. We registered the increasing signal of AI from caput to cauda epididymis. We confirmed that using gene expression method. Using specific antibody, AI was localized in the secretory tissues of caput, corpus and cauda epididymis by IMF. (en)
Title	Study of acrosin inhibitor along the boar epididymis Study of acrosin inhibitor along the boar epididymis (en)
skos:prefLabel	Study of acrosin inhibitor along the boar epididymis Study of acrosin inhibitor along the boar epididymis (en)
skos:notation	RIV/86652036:_____/10:00349095!RIV11-GA0-86652036
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06011), P(GA303/09/1285), P(GD523/08/H064), Z(AV0Z50520701)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Jonáková, Věra Cozlová, Nina Maňásková-Postlerová, Pavla Dorosh, Andriy
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	290730
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/10:00349095
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	spermatozoa; acrosin inhibitor; proteolytic enzymes; glycosidic enzyme; glycoproteins; antibody (en)
http://linked.open.../riv/klicoveSlovo	glycoproteins antibody spermatozoa acrosin inhibitor glycosidic enzyme proteolytic enzymes
http://linked.open...ontrolniKodProRIV	[30E02FED649C]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment. Study of the sperm membrane proteins with zona pellucida binding activity Biotechnologies of mammalian gametes
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Davidová, Nina Jonáková, Věra Postlerová, Pavla Dorosh, Andryi
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR

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