About: Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices     Goto   Sponge   NotDistinct   Permalink

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  • Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation of GMO. Here we used Affymetrix microarray platform to evaluate transcriptional differences between commercial MON810 GM maize and non-transgenic crops. We took two common MON810/non-GM variety pairs and two farming practices. MON810 and comparable non-GM varieties have very low numbers of sequences with differential expression. We show, the differences between a given MON810 variety and the non-GM do not depend on the assayed cultural conditions. Natural variation explained most of the variability in gene expression. Up to 37 % was dependent upon the variety and 32 % a result of the fertilization. The MON810 had minor effect (9.7%) on gene expression. This indicates that transcriptional differences of conventionally-bred varieties and different environmental conditions should be taken into account in safety assessment studies of GM plants.
  • Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation of GMO. Here we used Affymetrix microarray platform to evaluate transcriptional differences between commercial MON810 GM maize and non-transgenic crops. We took two common MON810/non-GM variety pairs and two farming practices. MON810 and comparable non-GM varieties have very low numbers of sequences with differential expression. We show, the differences between a given MON810 variety and the non-GM do not depend on the assayed cultural conditions. Natural variation explained most of the variability in gene expression. Up to 37 % was dependent upon the variety and 32 % a result of the fertilization. The MON810 had minor effect (9.7%) on gene expression. This indicates that transcriptional differences of conventionally-bred varieties and different environmental conditions should be taken into account in safety assessment studies of GM plants. (en)
Title
  • Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices
  • Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices (en)
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  • Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices
  • Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices (en)
skos:notation
  • RIV/86652036:_____/10:00346926!RIV11-AV0-86652036
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 273873
http://linked.open...ai/riv/idVysledku
  • RIV/86652036:_____/10:00346926
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • GMO (Genetically Modified Organism); MON810; Maize; Transcriptome (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [04B45CDC6B43]
http://linked.open...i/riv/nazevZdroje
  • Plant Molecular Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 73
http://linked.open...iv/tvurceVysledku
  • Kubista, Mikael
  • Pla, M.
  • Capellades, G.
  • Coll, A.
  • Collado, R.
  • Messeguer, J.
  • Nadal, A.
http://linked.open...ain/vavai/riv/wos
  • 000276911800009
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-4412
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