About: Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	qPCR tomography was developed to study mRNA localization in complex biological samples that are embedded and cryo-sectioned. After total RNA extraction and reverse transcription, the spatial profiles of mRNAs and other functional RNAs were determined by qPCR. The Xenopus laevis oocyte was selected as model, because of its large size (more than 1 mm) and large amount of total RNA ( 5 μg). Fifteen sections along the animal–vegetal axis were cut and prepared for quantification of 31 RNA targets using the high-throughput real-time RT-PCR (qPCR) BioMark platform. mRNAs were found to have two localization patterns, animal/central or vegetal. Because of the high resolution in sectioning, it was possible to distinguish two subgroups of the vegetal gene patterns: germ plasm determinant pattern and profile of other vegetal genes qPCR tomography was developed to study mRNA localization in complex biological samples that are embedded and cryo-sectioned. After total RNA extraction and reverse transcription, the spatial profiles of mRNAs and other functional RNAs were determined by qPCR. The Xenopus laevis oocyte was selected as model, because of its large size (more than 1 mm) and large amount of total RNA ( 5 μg). Fifteen sections along the animal–vegetal axis were cut and prepared for quantification of 31 RNA targets using the high-throughput real-time RT-PCR (qPCR) BioMark platform. mRNAs were found to have two localization patterns, animal/central or vegetal. Because of the high resolution in sectioning, it was possible to distinguish two subgroups of the vegetal gene patterns: germ plasm determinant pattern and profile of other vegetal genes (en)
Title	Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography (en)
skos:prefLabel	Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography (en)
skos:notation	RIV/86652036:_____/10:00346483!RIV11-GA0-86652036
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/09/1752), P(IAA500970904), Z(AV0Z50520701), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kubista, Mikael Švec, David Šídová, Monika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	288965
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/10:00346483
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Xenopus; Oocyte; qPCR tomography (en)
http://linked.open.../riv/klicoveSlovo	Oocyte Xenopus qPCR tomography
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D45F9BFBB2FC]
http://linked.open...i/riv/nazevZdroje	Methods
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	MicroRNA expression during early development of Xenopus laevis and mRNA degradation Formation of spatiotemporal molecular gradients in early development of Xenopus laevis
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Kubista, Mikael Švec, David Šídová, Monika Šindelka, R.
http://linked.open...ain/vavai/riv/wos	000277953300013
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR Signaling and cellular response mechanisms
issn	1046-2023
number of pages	5 (xsd:int)

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