About: Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization     Goto   Sponge   NotDistinct   Permalink

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  • Ultrastructure of sterlet Acipenser ruthenus L. 1758 sperm was examined by scanning and transmission electron microscopy, which allowed us to use various methods for visualizations of different parts of sterlet spermatozoa. Sperm cells possess a head with a distinct acrosome, a midpiece and a single flagellum surrounded by the flagellar plasma membrane. The average length of the head including the acrosome and the midpiece was estimated as 5.14 +/- 0.42 mu m. Nine to 10 posterolateral projections were derived from the acrosome. Three inter-twining endonuclear canals bounded by membranes traversed the nucleus in its whole length from the acrosome to the implantation fossa. Acrosin was located in all the three parts (acrosome, endonuclear canals and implantation fossa). The proximal and distal centrioles located in the midpiece compacted of nine peripheral triplets of microtubules. One cut of the midpiece contained from two to six mitochondria with area of 215 +/- 85 nm(2) in average. The flagellum was 42.47 +/- 1.89 mu m in length with typical eukaryotic organization of one central pair and nine peripheral pairs of microtubules. It passed through a cytoplasmic channel in the midpiece, which was formed by an invagination at the plasmallemma. The flagellum gradually developed two lateral extensions of its plasma membrane, so-called %22fins%22. Detected morphological variation can be described by four principal component axes corresponding to groups of individual morphometric characters defined on the sperm structures. Correlations among the characters indicate that the sperms are variable in their shape rather than size. Significant variation among examined fish individuals was found only in flagellum and nucleus length. Comparison between the present and previous studies of morphology of sturgeon spermatozoa confirmed large inter- and/or intra-specific differences that could be of substantial taxonomic value. (C) 2008 Elsevier B.V. All rights reserved.
  • Ultrastructure of sterlet Acipenser ruthenus L. 1758 sperm was examined by scanning and transmission electron microscopy, which allowed us to use various methods for visualizations of different parts of sterlet spermatozoa. Sperm cells possess a head with a distinct acrosome, a midpiece and a single flagellum surrounded by the flagellar plasma membrane. The average length of the head including the acrosome and the midpiece was estimated as 5.14 +/- 0.42 mu m. Nine to 10 posterolateral projections were derived from the acrosome. Three inter-twining endonuclear canals bounded by membranes traversed the nucleus in its whole length from the acrosome to the implantation fossa. Acrosin was located in all the three parts (acrosome, endonuclear canals and implantation fossa). The proximal and distal centrioles located in the midpiece compacted of nine peripheral triplets of microtubules. One cut of the midpiece contained from two to six mitochondria with area of 215 +/- 85 nm(2) in average. The flagellum was 42.47 +/- 1.89 mu m in length with typical eukaryotic organization of one central pair and nine peripheral pairs of microtubules. It passed through a cytoplasmic channel in the midpiece, which was formed by an invagination at the plasmallemma. The flagellum gradually developed two lateral extensions of its plasma membrane, so-called %22fins%22. Detected morphological variation can be described by four principal component axes corresponding to groups of individual morphometric characters defined on the sperm structures. Correlations among the characters indicate that the sperms are variable in their shape rather than size. Significant variation among examined fish individuals was found only in flagellum and nucleus length. Comparison between the present and previous studies of morphology of sturgeon spermatozoa confirmed large inter- and/or intra-specific differences that could be of substantial taxonomic value. (C) 2008 Elsevier B.V. All rights reserved. (en)
Title
  • Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization
  • Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization (en)
skos:prefLabel
  • Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization
  • Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization (en)
skos:notation
  • RIV/86652036:_____/09:00381935!RIV13-AV0-86652036
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA524/06/0817), S, Z(AV0Z50520514), Z(AV0Z50520701), Z(AV0Z60220518), Z(MSM6007665809)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 315077
http://linked.open...ai/riv/idVysledku
  • RIV/86652036:_____/09:00381935
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Acipenser ruthenus; Acrosome; Acrosin; Morphometric analysis; SEM; TEM (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [9DA6F87169C0]
http://linked.open...i/riv/nazevZdroje
  • Animal Reproduction Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 111
http://linked.open...iv/tvurceVysledku
  • Těšitel, J.
  • Linhart, O.
  • Pšenička, M.
  • Koubek, Pavel
  • Vančová, M.
http://linked.open...ain/vavai/riv/wos
  • 000262681600002
http://linked.open...n/vavai/riv/zamer
issn
  • 0378-4320
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.anireprosci.2008.02.006
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