About: Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recognition and binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. This is the topic of many scientific groups, and so far many molecules on the sperm surface, involved in mentioned process, were identified. Sperm surface proteins of ejaculated and capacitated boar sperms were isolated by Pierce Cell Surface Isolation Kit and characterized by 1D, 2D electrophoreses, glycoprotein staining, hyaluronidase and proteolytic activities. Finally binding assay with ZP was performed. Results have shown the quantitative and qualitative differences in both 1D and 2D electrophoretic profiles, glycoprotein profile was similar to 1D electrophoretic one. Neither hyaluronidase nor proteolytic activities were observed. Binding assay proved that interactions occur between both ejaculated and capacitated sperms. Protein interactions of capacitated sperm with ZP glycoproteins seem to be more specific. Recognition and binding of spermatozoa to the zona pellucida (ZP) is a crucial step in the fertilization process. This is the topic of many scientific groups, and so far many molecules on the sperm surface, involved in mentioned process, were identified. Sperm surface proteins of ejaculated and capacitated boar sperms were isolated by Pierce Cell Surface Isolation Kit and characterized by 1D, 2D electrophoreses, glycoprotein staining, hyaluronidase and proteolytic activities. Finally binding assay with ZP was performed. Results have shown the quantitative and qualitative differences in both 1D and 2D electrophoretic profiles, glycoprotein profile was similar to 1D electrophoretic one. Neither hyaluronidase nor proteolytic activities were observed. Binding assay proved that interactions occur between both ejaculated and capacitated sperms. Protein interactions of capacitated sperm with ZP glycoproteins seem to be more specific. (en)
Title	Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins (en)
skos:prefLabel	Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins Isolation and characterization of the sperm surface proteins and their binding studies with zona pellucida glycoproteins (en)
skos:notation	RIV/86652036:_____/09:00330614!RIV10-MSM-86652036
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06011), P(GA303/09/1285), Z(AV0Z50520701)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Jonáková, Věra Maňásková-Postlerová, Pavla Zigo, Michal
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	320503
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/09:00330614
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	zona pellucida; boar spermatozoa; glycoproteins; hyaluronidase (en)
http://linked.open.../riv/klicoveSlovo	glycoproteins hyaluronidase boar spermatozoa zona pellucida
http://linked.open...ontrolniKodProRIV	[62F6780DCDE3]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment. Study of the sperm membrane proteins with zona pellucida binding activity
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Jonáková, Věra Zigo, Michal Postlerová, Pavla
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 75 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software