About: Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry

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About: Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Washed red blood cells (RBCs), supplemented or non-supplemented with sodium azide (to inhibit catalase activity), were exposed to different concentrations of hydrogen peroxide as well as ascorbic acid. Strikingly, catalase within RBCs protected the cells against exogenic hydrogen peroxide even at millimolar concentrations. However, the activity of the erythrocytic catalase failed to protect the RBCs when they were exposed to an oxidative burst of stimulated polymorphonuclear cells (PMNCs) in the presence of several reactive species in addition to peroxide. Oxyhemoglobin, with an excess of hydrogen peroxide, formed oxidized hemoglobin species and caused protein denaturation as well as the rise of heme degradation products which was suspected to falsify zinc protoporphyrin/heme (ZPP/heme) ratio as assessed by hematofluorometry. Our experiments may thus imply that the non-fluorescent hemoglobin background can be modified by reactive oxygen species (ROS) and this can lead to a spurious ZPP/heme ratio. Washed red blood cells (RBCs), supplemented or non-supplemented with sodium azide (to inhibit catalase activity), were exposed to different concentrations of hydrogen peroxide as well as ascorbic acid. Strikingly, catalase within RBCs protected the cells against exogenic hydrogen peroxide even at millimolar concentrations. However, the activity of the erythrocytic catalase failed to protect the RBCs when they were exposed to an oxidative burst of stimulated polymorphonuclear cells (PMNCs) in the presence of several reactive species in addition to peroxide. Oxyhemoglobin, with an excess of hydrogen peroxide, formed oxidized hemoglobin species and caused protein denaturation as well as the rise of heme degradation products which was suspected to falsify zinc protoporphyrin/heme (ZPP/heme) ratio as assessed by hematofluorometry. Our experiments may thus imply that the non-fluorescent hemoglobin background can be modified by reactive oxygen species (ROS) and this can lead to a spurious ZPP/heme ratio. (en)
Title	Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry (en)
skos:prefLabel	Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry Oxidative stress may modify zinc protoporphyrin/heme ratio in hematofluorometry (en)
skos:notation	RIV/75010330:_____/10:00008185!RIV11-MZ0-75010330
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(NS9802)
http://linked.open...iv/cisloPeriodika	1p2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Janoušek, Stanislav Jírová, Dagmar Kejlová, Kristýna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Státní zdravotní ústav, příspěvková organizace
http://linked.open...dnocenehoVysledku	277879
http://linked.open...ai/riv/idVysledku	RIV/75010330:_____/10:00008185
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	oxidative stress; zinc-protoporphyrin (en)
http://linked.open.../riv/klicoveSlovo	oxidative stress zinc-protoporphyrin
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[DC904213FD73]
http://linked.open...i/riv/nazevZdroje	International journal of laboratory hematology
http://linked.open...in/vavai/riv/obor	FN
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Semilongitudinal study of somatic development and injury incidence in school children
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Jírová, Dagmar Kejlová, Kristina Janoušek, Stanislav Janoušek, S. J. Rosa, L.
http://linked.open...ain/vavai/riv/wos	000275511300006
issn	1751-5521
number of pages	10 (xsd:int)

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