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| Description
| - Although the biological applications of polyaniline are on the rise, they are limited to a certain extent by its insolubility in aqueous media. It is commonly known that conducting polyaniline is poorly soluble even in organic solvents, which strongly influences its processability. The preparation of conducting polymer colloids is one of possible approach to coping with this problem. Cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. The antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for Bacillus cereus and Escherichia coli, with a minimum inhibitory concentration of 3500 µg mL-1. The data recorded on human keratinocyte and a mouse embryonic fibroblast cell lines using an MTT assay and flow cytometry indicated a concentration-dependent cytotoxicity of colloid, with an absence of cytotoxic effect at around of 150 µg mL 1. The neutrophil oxidative burst test then showed that colloidal polyaniline, in concentrations < 150 µg mL-1, was not able to stimulate the production of reactive oxygen species in neutrophils and whole human blood. However, it worked efficiently as a scavenger of those already formed. The combination and mutual assessment of obtained results suggests that the conducting polyaniline colloid possesses low cytotoxicity and an absence of an oxidative burst, however a weak antibacterial performance should be considered a known limitation.
- Although the biological applications of polyaniline are on the rise, they are limited to a certain extent by its insolubility in aqueous media. It is commonly known that conducting polyaniline is poorly soluble even in organic solvents, which strongly influences its processability. The preparation of conducting polymer colloids is one of possible approach to coping with this problem. Cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. The antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for Bacillus cereus and Escherichia coli, with a minimum inhibitory concentration of 3500 µg mL-1. The data recorded on human keratinocyte and a mouse embryonic fibroblast cell lines using an MTT assay and flow cytometry indicated a concentration-dependent cytotoxicity of colloid, with an absence of cytotoxic effect at around of 150 µg mL 1. The neutrophil oxidative burst test then showed that colloidal polyaniline, in concentrations < 150 µg mL-1, was not able to stimulate the production of reactive oxygen species in neutrophils and whole human blood. However, it worked efficiently as a scavenger of those already formed. The combination and mutual assessment of obtained results suggests that the conducting polyaniline colloid possesses low cytotoxicity and an absence of an oxidative burst, however a weak antibacterial performance should be considered a known limitation. (en)
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| Title
| - Colloidal polyaniline dispersions: Antibacterial activity, cytotoxicity and neutrophil oxidative burst
- Colloidal polyaniline dispersions: Antibacterial activity, cytotoxicity and neutrophil oxidative burst (en)
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| skos:prefLabel
| - Colloidal polyaniline dispersions: Antibacterial activity, cytotoxicity and neutrophil oxidative burst
- Colloidal polyaniline dispersions: Antibacterial activity, cytotoxicity and neutrophil oxidative burst (en)
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| skos:notation
| - RIV/70883521:28610/14:43872570!RIV15-GA0-28610___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/70883521:28610/14:43872570
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Oxidative burst; Necrosis; Cytotoxicity; Colloidal polyaniline; Apoptosis; Antibacterial activity (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Humpolíček, Petr
- Kašpárková, Věra
- Kuceková, Zdenka
- Stejskal, Jaroslav
- Sáha, Petr
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| http://localhost/t...ganizacniJednotka
| |
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