About: Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment     Goto   Sponge   NotDistinct   Permalink

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  • Collagen films (Atelocollagen from bovine Achilles tendon) were prepared in tissue culture dishes and their surfaces were modified by using air and nitrogen plasma treatment. The treated samples were characterised by surface probe techniques including attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy imaging (SEM) and X-ray photoelectron spectroscopy (XPS). In addition, human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seeded on the treated and untreated films and cell proliferation was measured by using MTT assay test. The characterisation results confirmed physical and chemical changes on the collagen surface, such as increase in the extent of surface oxidation and surface roughness as well as, the treated samples showed better cell growth than untreated ones, and therefore this approach may be taken into account in the development of promising materials for tissue regeneration applications.
  • Collagen films (Atelocollagen from bovine Achilles tendon) were prepared in tissue culture dishes and their surfaces were modified by using air and nitrogen plasma treatment. The treated samples were characterised by surface probe techniques including attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy imaging (SEM) and X-ray photoelectron spectroscopy (XPS). In addition, human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seeded on the treated and untreated films and cell proliferation was measured by using MTT assay test. The characterisation results confirmed physical and chemical changes on the collagen surface, such as increase in the extent of surface oxidation and surface roughness as well as, the treated samples showed better cell growth than untreated ones, and therefore this approach may be taken into account in the development of promising materials for tissue regeneration applications. (en)
Title
  • Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment
  • Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment (en)
skos:prefLabel
  • Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment
  • Enhanced keratinocyte cell attachment to atelocollagen thin films through air and nitrogen plasma treatment (en)
skos:notation
  • RIV/70883521:28610/11:43865491!RIV12-MSM-28610___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED2.1.00/03.0111), S, Z(MSM7088352101)
http://linked.open...iv/cisloPeriodika
  • Neuvedeno
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 197599
http://linked.open...ai/riv/idVysledku
  • RIV/70883521:28610/11:43865491
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Atelocollagen,Thin films,Plasma treatment,Cell adhesion,Cell growth,Radio frequency discharge (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [DB44C4FC0FAD]
http://linked.open...i/riv/nazevZdroje
  • Progress in Colloid and Polymer Science
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http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 138
http://linked.open...iv/tvurceVysledku
  • Humpolíček, Petr
  • Junkar, Ita
  • Lehocký, Marián
  • López García, Jorge Andrés
  • Pacherník, Jiří
  • Sáha, Petr
http://linked.open...n/vavai/riv/zamer
issn
  • 0340-255X
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/978-3-642-19038-4_16
http://localhost/t...ganizacniJednotka
  • 28610
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