About: Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito

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About: Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0944501313001717
Description	Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fingerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiens mosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The optimal in vitro growth temperature of the strain was 33°C. Strain BR91 was sensitive to trimethoprim, nalidixic acid, 5-fluorouracil, and tolerated phosphomycin. The strain produced acid and alkaline phosphatase, esterase (C4), esterase-lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and α-fucosidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay revealed several major proteins in the size range of 13-16kDa, 22-30kDa and 37-131kDa. Fatty acid methyl ester (FAME) analysis showed that C14:0, C16:0, C18:1 ω9c and summed feature 5 (C18:2 ω6,9c and/or C18:0 anteiso) are major fatty acids. This study highlights certain phenotypic differences between strain BR91 and the Lyme disease spirochaete Borrelia burgdorferi, and supports the hypothesis that strain BR91 represents a unique taxonomical entity in a system of spirochaetal species. Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fingerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiens mosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The optimal in vitro growth temperature of the strain was 33°C. Strain BR91 was sensitive to trimethoprim, nalidixic acid, 5-fluorouracil, and tolerated phosphomycin. The strain produced acid and alkaline phosphatase, esterase (C4), esterase-lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase and α-fucosidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay revealed several major proteins in the size range of 13-16kDa, 22-30kDa and 37-131kDa. Fatty acid methyl ester (FAME) analysis showed that C14:0, C16:0, C18:1 ω9c and summed feature 5 (C18:2 ω6,9c and/or C18:0 anteiso) are major fatty acids. This study highlights certain phenotypic differences between strain BR91 and the Lyme disease spirochaete Borrelia burgdorferi, and supports the hypothesis that strain BR91 represents a unique taxonomical entity in a system of spirochaetal species. (en)
Title	Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito (en)
skos:prefLabel	Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito (en)
skos:notation	RIV/70883521:28110/14:43871566!RIV15-MSM-28110___
http://linked.open...avai/riv/aktivita	I P V
http://linked.open...avai/riv/aktivity	I, P(GA206/00/1234), P(GA206/03/0726), V
http://linked.open...iv/cisloPeriodika	5-6
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Buňková, Leona
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Tomáše Bati ve Zlíně / Fakulta technologická
http://linked.open...dnocenehoVysledku	44266
http://linked.open...ai/riv/idVysledku	RIV/70883521:28110/14:43871566
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Culex pipiens, Enzyme activity, FAME, SDS-PAGE, Spirochaete (en)
http://linked.open.../riv/klicoveSlovo	FAME Enzyme activity Spirochaete SDS-PAGE Culex pipiens
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[D17E8CC4EE49]
http://linked.open...i/riv/nazevZdroje	Microbiological Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	The study of selected groups of haematophagous diptera as possible carriers of spirochaetes including pathogenic species Ecology of emerging arthropod-borne microorganisms
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	169
http://linked.open...iv/tvurceVysledku	Buňková, Leona Rudolf, Ivo Halouzka, Jiří Šikutová, Silvie Krejčí, Eva Sanogo, Yibayiri O.
http://linked.open...ain/vavai/riv/wos	000335107900003
issn	0944-5013
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.micres.2013.10.002
http://localhost/t...ganizacniJednotka	28110

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