| Attributes | Values |
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| rdf:type
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| Description
| - The overriding function of tissue engineering is to design biological substitutes for tissue/organ replacement. Within this framework, Collagen is considered as a primary source in biomedical application, since this protein holds an excellent biocompatibility, innocuousness and biodegradability. Contamination is a serious concern in tissue replacement. It is responsible for microorganisms and infections growth over the treated areas. Several implants have to be removed by their poor performances. In fact, nosocomial infections are the foremost worldwide cause of death and disability, which in terms of healthiness and money represents indeed a difficulty that has to be solved. It is a well-known fact that bacterial adhesion and colonisation on the outermost layers of materials are surface specific and highly influenced by surface properties.An efficient way to thwart this problem is by means of anti-infective materials. Therefore, the focus of this contribution was upon the preparation of atelocollagen substrates with the following cost-effective and widely tested biocides at different concentrations: Benzalkonium chloride, bronopol, chitosan, chlorhexidine and irgasan. In order to assess how these antibacterial agents influence keratinocytes cell growth on the prepared-substrates, cytotoxicity and cell proliferation were determined by using MTT assay. The data indicated a low toxicity employing any of these chemical substances. Furthermore, cell viability was comparatively similar to the specimens without biocides. It means that regardless of the agent, collagen-cell-attachment properties were not drastically affected by the incorporation of those into the substrates. These findings suggest that the samples referred to herein as %22antibacterial substrates%22 might be suitable for tissue engineering applications.
- The overriding function of tissue engineering is to design biological substitutes for tissue/organ replacement. Within this framework, Collagen is considered as a primary source in biomedical application, since this protein holds an excellent biocompatibility, innocuousness and biodegradability. Contamination is a serious concern in tissue replacement. It is responsible for microorganisms and infections growth over the treated areas. Several implants have to be removed by their poor performances. In fact, nosocomial infections are the foremost worldwide cause of death and disability, which in terms of healthiness and money represents indeed a difficulty that has to be solved. It is a well-known fact that bacterial adhesion and colonisation on the outermost layers of materials are surface specific and highly influenced by surface properties.An efficient way to thwart this problem is by means of anti-infective materials. Therefore, the focus of this contribution was upon the preparation of atelocollagen substrates with the following cost-effective and widely tested biocides at different concentrations: Benzalkonium chloride, bronopol, chitosan, chlorhexidine and irgasan. In order to assess how these antibacterial agents influence keratinocytes cell growth on the prepared-substrates, cytotoxicity and cell proliferation were determined by using MTT assay. The data indicated a low toxicity employing any of these chemical substances. Furthermore, cell viability was comparatively similar to the specimens without biocides. It means that regardless of the agent, collagen-cell-attachment properties were not drastically affected by the incorporation of those into the substrates. These findings suggest that the samples referred to herein as %22antibacterial substrates%22 might be suitable for tissue engineering applications. (en)
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| Title
| - HaCaT Keratinocytes Cell Growth on Antibacterial Atelocollagen Substrates
- HaCaT Keratinocytes Cell Growth on Antibacterial Atelocollagen Substrates (en)
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| skos:prefLabel
| - HaCaT Keratinocytes Cell Growth on Antibacterial Atelocollagen Substrates
- HaCaT Keratinocytes Cell Growth on Antibacterial Atelocollagen Substrates (en)
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| skos:notation
| - RIV/70883521:28110/12:43869610!RIV13-MSM-28110___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/70883521:28110/12:43869610
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - MTT assay; Atelocollagen, Antibacterial substrates, Keratinocytes (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Humpolíček, Petr
- Lehocký, Marián
- Sáha, Petr
- López García, Jorge Andres
|
| http://localhost/t...ganizacniJednotka
| |
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