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  • Caffeine contents were determined in 10 commercial coffee brands (5 Vietnamese and 5 Czech) using 3 different methods, including direct conventional UV/VIS spectrophotometry at 273.2 nm, 1st order derivative UV/VIS spectrophotometry of the sample solution using peak to peak method, and liquid-liquid extraction using chloroform (CHCl3) as an extracting solvent. Caffeine contents measured by conventional UV/VIS spectrophotometry fall out of the documented range (2.9 ± 0.05 - 15 ± 0.07 %) while those determined by derivative spectrophotometry and by CHCl3 were found to be within the documented range (0.48 ± 0.01 – 3.47 ± 0.08 %) in all coffee samples. The 1st order derivative UV/VIS spectrophotometry and CHCl3 extraction can be used for determination of caffeine in Czech coffee brands (2.16 ± 0.04 and 2.06 ± 0.06 %, respectively). However, there were significant differences in the results between the two methods in Vietnamese coffee brands due to interference of complex matrices (1.62 ± 0.02 and 1.23 ± 0.03 %, respectively).
  • Caffeine contents were determined in 10 commercial coffee brands (5 Vietnamese and 5 Czech) using 3 different methods, including direct conventional UV/VIS spectrophotometry at 273.2 nm, 1st order derivative UV/VIS spectrophotometry of the sample solution using peak to peak method, and liquid-liquid extraction using chloroform (CHCl3) as an extracting solvent. Caffeine contents measured by conventional UV/VIS spectrophotometry fall out of the documented range (2.9 ± 0.05 - 15 ± 0.07 %) while those determined by derivative spectrophotometry and by CHCl3 were found to be within the documented range (0.48 ± 0.01 – 3.47 ± 0.08 %) in all coffee samples. The 1st order derivative UV/VIS spectrophotometry and CHCl3 extraction can be used for determination of caffeine in Czech coffee brands (2.16 ± 0.04 and 2.06 ± 0.06 %, respectively). However, there were significant differences in the results between the two methods in Vietnamese coffee brands due to interference of complex matrices (1.62 ± 0.02 and 1.23 ± 0.03 %, respectively). (en)
Title
  • DETERMINATION OF CAFFEINE CONTENTS IN VIETNAMESE AND CZECH COFFEE
  • DETERMINATION OF CAFFEINE CONTENTS IN VIETNAMESE AND CZECH COFFEE (en)
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  • DETERMINATION OF CAFFEINE CONTENTS IN VIETNAMESE AND CZECH COFFEE
  • DETERMINATION OF CAFFEINE CONTENTS IN VIETNAMESE AND CZECH COFFEE (en)
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  • RIV/70883521:28110/12:43869408!RIV13-MSM-28110___
http://linked.open...avai/riv/aktivita
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  • 130560
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  • RIV/70883521:28110/12:43869408
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  • Vietnamese coffee, Czech coffee, caffeine, liquid-liquid extraction, derivative spectrophotometry, UV/VIS.natamycin, trvanlivé fermentované salámy, HPLC-MS (en)
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  • [7C2DA95F4056]
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  • Kráčmar, Stanislav
  • Kubáň, Vlastimil
  • Phan, Thi Thanh Dieu
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  • Slovenská poľnohospodárska univerzita v Nitre
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  • 978-80-552-0769-8
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  • 28110
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