About: Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences

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Description	TGGE is an efficient method which allows us to study both composition and dynamics of complex microbial communities without the necessity of cultivation. Sample´s total DNA isolation and consequent PCR amplification of desired gene´s fragment need to be carried out before the TGGE experiment. Such pretreatment provides us with the population of gene fragments of the identical length from virtually all the strains present in the sample, and differing in their exact sequences. The DNA sequence variation in obtained gene fragments is a base for subsequent TGGE separation and identification of particular bacterial strains. Currently an equivalent method called denaturing gradient gel electrophoresis (DGGE) is used more often usually performed on D-code instrument (Bio-rad). In our laboratory we try to adopt TGGE instrumented on TGGE-Maxi system (Biometra) where the procedure is different particularly in the gel preparation and some other parameters. The instrument nowadays is relatively rare in the centra TGGE is an efficient method which allows us to study both composition and dynamics of complex microbial communities without the necessity of cultivation. Sample´s total DNA isolation and consequent PCR amplification of desired gene´s fragment need to be carried out before the TGGE experiment. Such pretreatment provides us with the population of gene fragments of the identical length from virtually all the strains present in the sample, and differing in their exact sequences. The DNA sequence variation in obtained gene fragments is a base for subsequent TGGE separation and identification of particular bacterial strains. Currently an equivalent method called denaturing gradient gel electrophoresis (DGGE) is used more often usually performed on D-code instrument (Bio-rad). In our laboratory we try to adopt TGGE instrumented on TGGE-Maxi system (Biometra) where the procedure is different particularly in the gel preparation and some other parameters. The instrument nowadays is relatively rare in the centra (en)
Title	Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences (en)
skos:prefLabel	Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences Temperature gradient gel electrophoresis (TGGE) in study of environmental samples: first experiences (en)
skos:notation	RIV/70883521:28110/10:63510070!RIV11-GA0-28110___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GAP108/10/0200)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Buňková, Leona Koutný, Marek Husárová, Lucie
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Tomáše Bati ve Zlíně / Fakulta technologická
http://linked.open...dnocenehoVysledku	292200
http://linked.open...ai/riv/idVysledku	RIV/70883521:28110/10:63510070
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	isolation of DNA; PCR; TGGE; reamplification of DNA (en)
http://linked.open.../riv/klicoveSlovo	reamplification of DNA isolation of DNA PCR TGGE
http://linked.open...ontrolniKodProRIV	[704E97C64196]
http://linked.open...v/mistoKonaniAkce	Stará Lesná, Vysoké Tatry
http://linked.open...i/riv/mistoVydani	Bratislava
http://linked.open...i/riv/nazevZdroje	25. výročný kongres československej spoločnosti mikrobiologickej s medzinárodnou účasťou
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biodegradation study of polymeric materials by combination of advanced methods
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Buňková, Leona Husárová, Lucie Koutný, Marek
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2010-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Československá spoločnosť mikrobiologická
https://schema.org/isbn	970-80-970477-8-8
http://localhost/t...ganizacniJednotka	28110

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