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  • A rapid, simple and reliable liquid chromatographic method has been developed for the determination of thiamin, nicotinamide, panthothenic acid and pyridoxine in pharmaceutical preparations. HPLC equipped with a reversed?phase C8 was used. A mobile phase of methanol-phosphate buffer (10:90, v/v) and a flow rate of 0.8 ml.min-1 gave the most satisfactory separation of those vitamins using a UV detector set up at 204 nm and 220 nm. Detection limits ranged from 2.0 to 12.0 ?g.ml-1. When the method was applied to the determination of the vitamins, the found values were lower or close with those on the labels. The differences in the tablet contents of vitamins may be influenced by the kind of pharmaceutical preparation, by technical preparation making process and by the storage conditions.
  • A rapid, simple and reliable liquid chromatographic method has been developed for the determination of thiamin, nicotinamide, panthothenic acid and pyridoxine in pharmaceutical preparations. HPLC equipped with a reversed?phase C8 was used. A mobile phase of methanol-phosphate buffer (10:90, v/v) and a flow rate of 0.8 ml.min-1 gave the most satisfactory separation of those vitamins using a UV detector set up at 204 nm and 220 nm. Detection limits ranged from 2.0 to 12.0 ?g.ml-1. When the method was applied to the determination of the vitamins, the found values were lower or close with those on the labels. The differences in the tablet contents of vitamins may be influenced by the kind of pharmaceutical preparation, by technical preparation making process and by the storage conditions. (en)
Title
  • The determination of water soluble vitramins in multivitamin pharmaceutical preparations
  • The determination of water soluble vitramins in multivitamin pharmaceutical preparations (en)
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  • The determination of water soluble vitramins in multivitamin pharmaceutical preparations
  • The determination of water soluble vitramins in multivitamin pharmaceutical preparations (en)
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  • RIV/70883521:28110/10:63509394!RIV11-MSM-28110___
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  • I, Z(MSM7088352101)
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  • 253840
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  • RIV/70883521:28110/10:63509394
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  • HPLC; thiamin; nicotinamide; pantothenic acid; pyridoxine; water soluble vitamin; pharmaceutical preparation (en)
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  • [B2E4DFDEC2D4]
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  • Bezpečnosť a kontrola potravín (Zborník prác z medzinárodnej vedeckej konferencie)
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  • Kožáková, Zuzana
  • Kramářová, Daniela
  • Rop, Otakar
  • Altangerel, Bayanmunkh
  • Hoza, Ignác
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  • Slovenská poľnohospodárska univerzita v Nitre
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  • 978-80-552-0350-8
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  • 28110
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