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Description
  • Work with dangerous pathogens pose risk of infection to the laboratory staff. Therefore, it would be suitable to inactivate the samples prior to their processing and thus minimise risk for infection. Majority of the commercial kits, however, does not fit to thermal treatment of samples. Therefore, the report deals, on the one hand, with modifications of isolation procedures and, on the other hand, it mainly monitors finding of the most efficient treatment of the samples for the detection of bacterial pathogens by means of real-time PCR after their thermal inactivation.
  • Work with dangerous pathogens pose risk of infection to the laboratory staff. Therefore, it would be suitable to inactivate the samples prior to their processing and thus minimise risk for infection. Majority of the commercial kits, however, does not fit to thermal treatment of samples. Therefore, the report deals, on the one hand, with modifications of isolation procedures and, on the other hand, it mainly monitors finding of the most efficient treatment of the samples for the detection of bacterial pathogens by means of real-time PCR after their thermal inactivation. (en)
  • Při práci s nebezpečnými patogeny hrozí riziko infekce laboratorního personálu. Bylo by proto vhodné vzorky před zpracováním inaktivovat a tím minimalizovat riziko infekce. Většina komerčních souprav však není pro zpracování tepelně opracovaných vzorků vhodná. Práce se proto věnuje jednak modifikacemi izolačních postupů, ale zejména sleduje nalezení nejefektivnějšího zpracování vzorků pro detekci bakteriálních patogenů pomocí real-time PCR po jejich tepelné inaktivaci (cs)
Title
  • Comparison of Different Procedures of Sample Processing in Relation to the Sensitivity of PCR Pathogen Detection
  • Comparison of Different Procedures of Sample Processing in Relation to the Sensitivity of PCR Pathogen Detection (en)
  • Porovnání různých postupů zpracování vzorků ve vztahu k citlivosti PCR detekce patogenů. (cs)
skos:prefLabel
  • Comparison of Different Procedures of Sample Processing in Relation to the Sensitivity of PCR Pathogen Detection
  • Comparison of Different Procedures of Sample Processing in Relation to the Sensitivity of PCR Pathogen Detection (en)
  • Porovnání různých postupů zpracování vzorků ve vztahu k citlivosti PCR detekce patogenů. (cs)
skos:notation
  • RIV/70565813:_____/08:#0000176!RIV09-SUJ-70565813
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAAX00310701), Z(SUJ200401)
http://linked.open...iv/cisloPeriodika
  • S2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 360570
http://linked.open...ai/riv/idVysledku
  • RIV/70565813:_____/08:#0000176
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • real-time PCR; Detection of pathogens; DNA isolation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [5D010F4F13D0]
http://linked.open...i/riv/nazevZdroje
  • Prague Medical report
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 109
http://linked.open...iv/tvurceVysledku
  • Rosenbergová, Kateřina
  • Kubíčková, Z.
  • Kubíček, Oldřich
http://linked.open...n/vavai/riv/zamer
issn
  • 1214-6994
number of pages
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