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Description
| - Measurements of pH are very important in medicine, biology, chemistry, environmental protection, etc. For such purposes both bulk measurement methods such as potentiometry and spectrophotometry, as well as different sensors including optical ones are used. The research of optical pH sensors is focused on novel absorption and luminescence pH transducers, pH-sensitive detection membranes, novel optical sensing structures, on the development of optical sensors making possible continuous pH detection in patient bodies or in plant and animal cells, etc. This thesis is focused on standard electrochemical and spectrophotometric bulk pH measurements and on characterizations of novel fiber-optic detection elements. It gives the necessary minimum to understand the physical and chemical nature of pH and its measurements and sensing. It also provides some information on existing measuring pH systems and their measuring methodics. Characterization experiments were carried out on U-shaped fiber-optic detection probes provided with porous detection layers immobilizing absorption pH indicator, methyl red. The probes have not been fabricated in the thesis. They were characterized by using reference solutions prepared in the thesis and measured with a glass pH electrode and VIS spectrophotometry. Spectral-response curves of the probes in a range of 350-800 nm were measured by using an experimental setup assembled in the thesis. Angular distributions of output power when excited probes by an inclined collimated beam and in some cases time-response curves of probes were also measured. Calibration curves of the probes were determined from which a pH working range of 4.5-6.5 with a maximum sensitivity of 0.03 1/pH unit were determined. One probe with suitable properties was chosen to perform qualitative measurements on real biological samples, namely urine and saliva.
- Measurements of pH are very important in medicine, biology, chemistry, environmental protection, etc. For such purposes both bulk measurement methods such as potentiometry and spectrophotometry, as well as different sensors including optical ones are used. The research of optical pH sensors is focused on novel absorption and luminescence pH transducers, pH-sensitive detection membranes, novel optical sensing structures, on the development of optical sensors making possible continuous pH detection in patient bodies or in plant and animal cells, etc. This thesis is focused on standard electrochemical and spectrophotometric bulk pH measurements and on characterizations of novel fiber-optic detection elements. It gives the necessary minimum to understand the physical and chemical nature of pH and its measurements and sensing. It also provides some information on existing measuring pH systems and their measuring methodics. Characterization experiments were carried out on U-shaped fiber-optic detection probes provided with porous detection layers immobilizing absorption pH indicator, methyl red. The probes have not been fabricated in the thesis. They were characterized by using reference solutions prepared in the thesis and measured with a glass pH electrode and VIS spectrophotometry. Spectral-response curves of the probes in a range of 350-800 nm were measured by using an experimental setup assembled in the thesis. Angular distributions of output power when excited probes by an inclined collimated beam and in some cases time-response curves of probes were also measured. Calibration curves of the probes were determined from which a pH working range of 4.5-6.5 with a maximum sensitivity of 0.03 1/pH unit were determined. One probe with suitable properties was chosen to perform qualitative measurements on real biological samples, namely urine and saliva. (en)
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Title
| - DETECTION OF PH WITH FIBER-OPTIC DETECTION ELEMENTS
- DETECTION OF PH WITH FIBER-OPTIC DETECTION ELEMENTS (en)
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skos:prefLabel
| - DETECTION OF PH WITH FIBER-OPTIC DETECTION ELEMENTS
- DETECTION OF PH WITH FIBER-OPTIC DETECTION ELEMENTS (en)
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skos:notation
| - RIV/68407700:21460/13:00213953!RIV14-MSM-21460___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68407700:21460/13:00213953
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - pH measurement; U-shaped fiber-optic probe; porous detection membrane; methyl red, spectral response curve; urine; saliva (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
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http://localhost/t...ganizacniJednotka
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