About: Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence     Goto   Sponge   NotDistinct   Permalink

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  • The whole-cell biosensor which used a special optical fiber element (OFE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector. The sensitivity depends on a value of detected bioluminescence (BL), which can be very low. In this study, we explore the influence of OFE shape on BL transition as well as number of cells immobilized into the active layer on detecting low-level bioluminescent signals. The bioluminescence signal was produced by the genetically engineered bacterial bioreporter (light source). Mathematical model of OFE shape was developed based on geometric optics. Four different shapes of OFE were studied experimentally. For development of mathematical model of the optimal OFE shape from point of view maximum detected intensity of BLwas applied parameters of the best OFE from experimental measurements. The correspondence between results of calculated transmission by the model and experimental measurements of BL was favourable.
  • The whole-cell biosensor which used a special optical fiber element (OFE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector. The sensitivity depends on a value of detected bioluminescence (BL), which can be very low. In this study, we explore the influence of OFE shape on BL transition as well as number of cells immobilized into the active layer on detecting low-level bioluminescent signals. The bioluminescence signal was produced by the genetically engineered bacterial bioreporter (light source). Mathematical model of OFE shape was developed based on geometric optics. Four different shapes of OFE were studied experimentally. For development of mathematical model of the optimal OFE shape from point of view maximum detected intensity of BLwas applied parameters of the best OFE from experimental measurements. The correspondence between results of calculated transmission by the model and experimental measurements of BL was favourable. (en)
Title
  • Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence
  • Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence (en)
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  • Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence
  • Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence (en)
skos:notation
  • RIV/68407700:21460/12:00202769!RIV13-MSM-21460___
http://linked.open...avai/riv/aktivita
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  • P(EE.2.3.20.0092)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 180957
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  • RIV/68407700:21460/12:00202769
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  • biosensor; GMO; microbial cell; bioluminiscence (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [E3155198BDE4]
http://linked.open...v/mistoKonaniAkce
  • Kladno
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  • Prague
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  • Instuments and Methods for Biology and Medicine 2012
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Jiřina, Marcel
  • Kalábová, Hana
  • Kuncová, G.
  • Pospíšilová, Marie
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
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  • České vysoké učení technické v Praze
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  • 978-80-01-05119-1
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  • 21460
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