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  • With the rise of antibiotic resistance of pathogenic bacteria there is an increased demand for monitoring the functionality of bacteria membranes, the disruption of which can be induced by peptide-lipid interactions. In this work we attempt to construct and disrupt supported lipid membranes (SLB) on boron doped nanocrystalline diamond (B-NCD). Electrochemical Impedance Spectroscopy (EIS) was used to study in situ changes related to lipid membrane formation and disruption by peptide-induced interactions. The observed impedance changes were minimal for oxidized B-NCD samples, but were still detectable in the low frequency part of the spectra. The sensitivity for the detection of membrane formation and disruption was significantly higher for hydrogenated B-NCD surfaces. Data modeling indicates large changes in the electrical charge when an electrical double layer is formed at the B-NCD/SLB interface, governed by ion absorption. By contrast, for oxidized B-NCD surfaces
  • With the rise of antibiotic resistance of pathogenic bacteria there is an increased demand for monitoring the functionality of bacteria membranes, the disruption of which can be induced by peptide-lipid interactions. In this work we attempt to construct and disrupt supported lipid membranes (SLB) on boron doped nanocrystalline diamond (B-NCD). Electrochemical Impedance Spectroscopy (EIS) was used to study in situ changes related to lipid membrane formation and disruption by peptide-induced interactions. The observed impedance changes were minimal for oxidized B-NCD samples, but were still detectable in the low frequency part of the spectra. The sensitivity for the detection of membrane formation and disruption was significantly higher for hydrogenated B-NCD surfaces. Data modeling indicates large changes in the electrical charge when an electrical double layer is formed at the B-NCD/SLB interface, governed by ion absorption. By contrast, for oxidized B-NCD surfaces (en)
Title
  • Boron Doped Nanocrystalline Diamond Films for Biosensing Applications
  • Boron Doped Nanocrystalline Diamond Films for Biosensing Applications (en)
skos:prefLabel
  • Boron Doped Nanocrystalline Diamond Films for Biosensing Applications
  • Boron Doped Nanocrystalline Diamond Films for Biosensing Applications (en)
skos:notation
  • RIV/68407700:21460/11:00183657!RIV12-MSM-21460___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LD11078), S, Z(MSM6840770012)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 188640
http://linked.open...ai/riv/idVysledku
  • RIV/68407700:21460/11:00183657
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • biosensor; nanocrystalline diamond; electrochemical impedance spectroscopy (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [0D63AD9EB04A]
http://linked.open...i/riv/nazevZdroje
  • Acta Polytechnica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 2011
http://linked.open...iv/tvurceVysledku
  • Krucky, J.
  • Petrák, Václav
  • Vlčková, Marie
http://linked.open...n/vavai/riv/zamer
issn
  • 1210-2709
number of pages
http://localhost/t...ganizacniJednotka
  • 21460
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