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Description
  • A confocal laser scanning microscope (CLSM) gives possibility to scan images from a biological specimen in different depths and obtain a stack of precisely registered fluorescent images within the specimen. Fluorescent image intensities from a CLSM suffer from several distortions. Particularly, images captured from deep layers of the specimen are often darker than images from the top most layers due to absorption and scattering of both excitation and fluorescent light. These effects cause problems in subsequent analysis of biological objects. Therefore, methods for compensation of the light attenuation with depth must be used.
  • A confocal laser scanning microscope (CLSM) gives possibility to scan images from a biological specimen in different depths and obtain a stack of precisely registered fluorescent images within the specimen. Fluorescent image intensities from a CLSM suffer from several distortions. Particularly, images captured from deep layers of the specimen are often darker than images from the top most layers due to absorption and scattering of both excitation and fluorescent light. These effects cause problems in subsequent analysis of biological objects. Therefore, methods for compensation of the light attenuation with depth must be used. (en)
  • Pokles jasu a kontrastu s hloubkou zaostření v konfokální mikroskopii se dá kompenzovat jak optimalizací mikroskopu během snímání, tak pomocí vhodných algoritmů zpracování dat po nasnímání. (cs)
Title
  • Efficient Compensation of the Light Attenuation with Depth in Confocal Microscopy
  • Účinná kompenzace útlumu jasu a kontrastu s hloubkou v konfokální mikroskopii (cs)
  • Efficient Compensation of the Light Attenuation with Depth in Confocal Microscopy (en)
skos:prefLabel
  • Efficient Compensation of the Light Attenuation with Depth in Confocal Microscopy
  • Účinná kompenzace útlumu jasu a kontrastu s hloubkou v konfokální mikroskopii (cs)
  • Efficient Compensation of the Light Attenuation with Depth in Confocal Microscopy (en)
skos:notation
  • RIV/68407700:21460/05:12117702!RIV09-MSM-21460___
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  • P(KJB6011309), P(ME 688), Z(AV0Z5011922), Z(MSM6840770012)
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  • 519658
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  • RIV/68407700:21460/05:12117702
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  • confocal microscopy; light attenuation (en)
http://linked.open.../riv/klicoveSlovo
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  • [C594A6598086]
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  • Praha
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  • Praha
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  • Proceedings of Workshop 2005 - Part A,B
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  • Čapek, Martin
  • Kubínová, L.
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number of pages
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  • České vysoké učení technické v Praze
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  • 80-01-03201-9
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  • 21460
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