About: Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy     Goto   Sponge   NotDistinct   Permalink

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Description
  • A confocal laser scanning microscope (CLSM) captures images from a biological specimen in different depths and provides one with a stack of precisely registered fluorescent images. However, image intensities suffer from light loss distortions showing contrast and brightness degradation of images with depth. This effect causes problems in subsequent analysis and segmentation of biological objects. Therefore, we developed a post-processing method to correct this problem. It is a two step algorithm. In the first step the algorithm determines a standard histogram of the stack of images and in the second step histograms of individual images are warped according to this standard histogram. The algorithm results in more stable brightness and contrast of images through the stack.
  • A confocal laser scanning microscope (CLSM) captures images from a biological specimen in different depths and provides one with a stack of precisely registered fluorescent images. However, image intensities suffer from light loss distortions showing contrast and brightness degradation of images with depth. This effect causes problems in subsequent analysis and segmentation of biological objects. Therefore, we developed a post-processing method to correct this problem. It is a two step algorithm. In the first step the algorithm determines a standard histogram of the stack of images and in the second step histograms of individual images are warped according to this standard histogram. The algorithm results in more stable brightness and contrast of images through the stack. (en)
  • Dvoustupňový algoritmus pro kompenzaci jasu a kontrastu s hloubkou zaostření v konfokální mikroskopii. (cs)
Title
  • Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy
  • Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy (en)
  • Kompenzace útlumu jasu a kontrastu obrazů s hloubkou zaostření v konfokální mikroskopii (cs)
skos:prefLabel
  • Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy
  • Compensation of the Contrast and Brightness Attenuation with Depth in Confocal Microscopy (en)
  • Kompenzace útlumu jasu a kontrastu obrazů s hloubkou zaostření v konfokální mikroskopii (cs)
skos:notation
  • RIV/68407700:21460/05:12108532!RIV09-MSM-21460___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAA100110502), P(IAA600110507), P(KJB6011309), Z(AV0Z50110509), Z(MSM6840770012)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 515969
http://linked.open...ai/riv/idVysledku
  • RIV/68407700:21460/05:12108532
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • brightness correction; contrast correction; histogram warping (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [7B3E6C33F4D8]
http://linked.open...v/mistoKonaniAkce
  • Budmerice
http://linked.open...i/riv/mistoVydani
  • Bratislava
http://linked.open...i/riv/nazevZdroje
  • Proceedings Spring Conference on Computer Graphics
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Čapek, Martin
  • Hána, Karel
  • Smrčka, Pavel
  • Kubínová, L.
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Univerzita Komenského v Bratislave
https://schema.org/isbn
  • 80-223-2057-9
http://localhost/t...ganizacniJednotka
  • 21460
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