About: Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation     Goto   Sponge   NotDistinct   Permalink

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Description
  • We introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluorescent samples of different thickness, labeling density and noise levels. The method provides good suppression of out of focus light, improves spatial resolution, and allows reconstruction of both 2D and 3D images of cells even in the case of weak signals. The method can be used to process both optical sectioning and super-resolution structured illumination microscopy data to create high quality super-resolution images.
  • We introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluorescent samples of different thickness, labeling density and noise levels. The method provides good suppression of out of focus light, improves spatial resolution, and allows reconstruction of both 2D and 3D images of cells even in the case of weak signals. The method can be used to process both optical sectioning and super-resolution structured illumination microscopy data to create high quality super-resolution images. (en)
Title
  • Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation
  • Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation (en)
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  • Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation
  • Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation (en)
skos:notation
  • RIV/68407700:21230/14:00221810!RIV15-MSM-21230___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(ED1.1.00/02.0109), P(GAP102/10/1320), P(GBP302/12/G157), P(GP14-15272P), P(GPP205/12/P392), P(LD12018), S
http://linked.open...iv/cisloPeriodika
  • 24
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 50489
http://linked.open...ai/riv/idVysledku
  • RIV/68407700:21230/14:00221810
http://linked.open...riv/jazykVysledku
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  • Fluorescence microscopy; Spatial light modulators; Image reconstruction techniques; Superresolution. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [6223662FE037]
http://linked.open...i/riv/nazevZdroje
  • Optics Express
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 22
http://linked.open...iv/tvurceVysledku
  • Fliegel, Karel
  • Klíma, Miloš
  • Křížek, P.
  • Hagen, G. M.
  • Švindrych, Z.
  • Lukeš, Tomáš
  • Benda, J.
  • Ovesný, M.
http://linked.open...ain/vavai/riv/wos
  • 000345770500048
issn
  • 1094-4087
number of pages
http://bibframe.org/vocab/doi
  • 10.1364/OE.22.029805
http://localhost/t...ganizacniJednotka
  • 21230
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