About: Compression tests of a living cell: a contact detection problem

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Alterations in the mechanical properties of cells are associated with cellular diversification and diseases. Assessment of mechanical properties of living cells was mostly associated with Atomic Force Microscopy technique. However, the contact conditions of AFM tip with cell’s membrane are very complex. Advantageously, very well defined geometry of flat end probe used for nanoindentation can simplify the contact problem into compression of an inflated spherical membrane by two rigid parallel planes. From our previous experiments we used COS-1 cells [ATCC, USA] because of their long viability and good adhesion properties. This cell line [ATCC code: CRL-1650] was derived from African green monkey kidney; the cells grow attached to the base (adherent) and have morphology of fibroblasts. The aims of present study are as follows: (1) Determination of the contact between flat punch tip and single cell and (2) to identify membrane bursting critical load. Alterations in the mechanical properties of cells are associated with cellular diversification and diseases. Assessment of mechanical properties of living cells was mostly associated with Atomic Force Microscopy technique. However, the contact conditions of AFM tip with cell’s membrane are very complex. Advantageously, very well defined geometry of flat end probe used for nanoindentation can simplify the contact problem into compression of an inflated spherical membrane by two rigid parallel planes. From our previous experiments we used COS-1 cells [ATCC, USA] because of their long viability and good adhesion properties. This cell line [ATCC code: CRL-1650] was derived from African green monkey kidney; the cells grow attached to the base (adherent) and have morphology of fibroblasts. The aims of present study are as follows: (1) Determination of the contact between flat punch tip and single cell and (2) to identify membrane bursting critical load. (en)
Title	Compression tests of a living cell: a contact detection problem Compression tests of a living cell: a contact detection problem (en)
skos:prefLabel	Compression tests of a living cell: a contact detection problem Compression tests of a living cell: a contact detection problem (en)
skos:notation	RIV/68407700:21220/14:00217609!RIV15-MSM-21220___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(LH12011), P(TA01010185), S
http://linked.open...iv/cisloPeriodika	17
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Lukeš, Jaroslav Šepitka, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	České vysoké učení technické v Praze / Fakulta strojní
http://linked.open...dnocenehoVysledku	8447
http://linked.open...ai/riv/idVysledku	RIV/68407700:21220/14:00217609
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nanoindentation; single cells; mechanical properties; Poroelastic, nanoDMA (en)
http://linked.open.../riv/klicoveSlovo	nanoDMA mechanical properties nanoindentation Poroelastic single cells
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[1220F968A6D8]
http://linked.open...i/riv/nazevZdroje	Computer Methods in Biomechanics and Biomedical Engineering
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New materials and coatings for joint replacement bionical design Structure of retroviral particle and intracellular transport of its components.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Lukeš, Jaroslav Šepitka, Josef Fuzik, T. Grznarová, P.
issn	1025-5842
number of pages	2 (xsd:int)
http://bibframe.org/vocab/doi	10.1080/10255842.2014.931093
http://localhost/t...ganizacniJednotka	21220

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