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  • The progress in the field of tissue engineering and clinical surgery demands further investigation of the correlation between mechanically exposed proteins of the extracellular matrix and the mechanical properties of macro-specimen. This paper discusses the microscopic method for the type I collagen and elastin fibre identification that effectively supports and explains biomechanical results. The examined object was vena saphena magna that is used for coronary artery bypass graft surgery. It has to be noted that the conditions in the venous and artery system differ significantly. The adopted mechanical testing was the inflation-extension test. The sample ofvena saphena was inflated four times up to the pressure 4 kPa (vein pressure) and then four times up to the 16 kPa (systolic pressure). The longitudinal and circumferential deformations of the tube were evaluated using the edge detection method. The second part of the same vein was immediately visualized for type I collagen by backward nondescanned second harmonic generation imaging and forelastin by twophoton excited autoflorescence. The result of the inflation-extension tests and the findings obtained during label-free optical imaging that requires little specimen processing for visualization of the mechanically most significant proteins, promises better understanding of the micro macro mechanical relationships.
  • The progress in the field of tissue engineering and clinical surgery demands further investigation of the correlation between mechanically exposed proteins of the extracellular matrix and the mechanical properties of macro-specimen. This paper discusses the microscopic method for the type I collagen and elastin fibre identification that effectively supports and explains biomechanical results. The examined object was vena saphena magna that is used for coronary artery bypass graft surgery. It has to be noted that the conditions in the venous and artery system differ significantly. The adopted mechanical testing was the inflation-extension test. The sample ofvena saphena was inflated four times up to the pressure 4 kPa (vein pressure) and then four times up to the 16 kPa (systolic pressure). The longitudinal and circumferential deformations of the tube were evaluated using the edge detection method. The second part of the same vein was immediately visualized for type I collagen by backward nondescanned second harmonic generation imaging and forelastin by twophoton excited autoflorescence. The result of the inflation-extension tests and the findings obtained during label-free optical imaging that requires little specimen processing for visualization of the mechanically most significant proteins, promises better understanding of the micro macro mechanical relationships. (en)
Title
  • Label-free optical methods for evaluation of mechanically exposed proteins in vein
  • Label-free optical methods for evaluation of mechanically exposed proteins in vein (en)
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  • Label-free optical methods for evaluation of mechanically exposed proteins in vein
  • Label-free optical methods for evaluation of mechanically exposed proteins in vein (en)
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  • RIV/68407700:21220/13:00206699!RIV14-MSM-21220___
http://linked.open...avai/riv/aktivita
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  • P(NT13302), S
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  • 84119
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  • RIV/68407700:21220/13:00206699
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  • tissue engineering; vein; vena; elastin; collagen; two photon excitation microscopy; SHG microscopy; label free; mechanical properties (en)
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  • [DD4D0BE31D64]
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  • Chlup, Hynek
  • Fanta, Ondřej
  • Jelen, Karel
  • Lopot, František
  • Veselý, Jan
  • Hadraba, Daniel
  • Burdikova, Zuzana
http://localhost/t...ganizacniJednotka
  • 21220
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