About: Termination pattern of nanocrystalline diamond controls cell behavior

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of the study was to investigate the behavior of human osteoblastic cells (SAOS-2) on nanocrystalline diamond (NCD) film with altered wettability with a focus on primary adhesion. Surface termination and thus wettability of the NCD was achieved by exposition to oxygen or hydrogen MW plasma. Two different surface termination patterns of the NCD were prepared: 1) half of the surface terminated by O and the other half by H, 2) surface with 200 um wide O/H alternating stripes. Differences in cell behavior caused by these various termination patterns were studied by live cell imaging phase contrast microscopy and subsequent image analysis based on image thresholding and cell tracking in NIS Elements software. Using these methods we determined the number of adherent and non-adherent cells and parameters of each cell migration track on the NCD samples with O/H halves and O/H stripes. The aim of the study was to investigate the behavior of human osteoblastic cells (SAOS-2) on nanocrystalline diamond (NCD) film with altered wettability with a focus on primary adhesion. Surface termination and thus wettability of the NCD was achieved by exposition to oxygen or hydrogen MW plasma. Two different surface termination patterns of the NCD were prepared: 1) half of the surface terminated by O and the other half by H, 2) surface with 200 um wide O/H alternating stripes. Differences in cell behavior caused by these various termination patterns were studied by live cell imaging phase contrast microscopy and subsequent image analysis based on image thresholding and cell tracking in NIS Elements software. Using these methods we determined the number of adherent and non-adherent cells and parameters of each cell migration track on the NCD samples with O/H halves and O/H stripes. (en)
Title	Termination pattern of nanocrystalline diamond controls cell behavior Termination pattern of nanocrystalline diamond controls cell behavior (en)
skos:prefLabel	Termination pattern of nanocrystalline diamond controls cell behavior Termination pattern of nanocrystalline diamond controls cell behavior (en)
skos:notation	RIV/68378271:_____/14:00434473!RIV15-GA0-68378271
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA14-04790S), P(GAP108/12/0996)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Kromka, Alexander Rezek, Bohuslav Babchenko, Oleg Ukraintsev, Egor
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	49970
http://linked.open...ai/riv/idVysledku	RIV/68378271:_____/14:00434473
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	diamond films; patterned surface; surface termination; cell adhesion (en)
http://linked.open.../riv/klicoveSlovo	diamond films patterned surface cell adhesion surface termination
http://linked.open...ontrolniKodProRIV	[820FAB314D3D]
http://linked.open...in/vavai/riv/obor	BM
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Bio-inspired Scaffolds as Active Sensor Systems Engineering Bulk and Surface of Diamond Nano-Objects for Biomedicine
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Babchenko, Oleg Brož, A. Kromka, Alexander Rezek, Bohuslav Ukraintsev, Egor Hubálek Kalbáčová, M. Ostrovská, L. Sauerová, P. Verdánová, M.

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