About: Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake.

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About: Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake. Goto Sponge NotDistinct Permalink

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Description	When studying non-transferrin-bound iron (NTBI) uptake by mammalian cells in the high-affinity range (micromolar and below), employment of strong chelators such as NTA can lead to kinetic artifacts due to inhibition by excess chelator. This can be avoided by using citrate at an excess concentration above as a little as1,5-fold over iron without danger of precipitation if balanced culture media are used as incubation mediumfor NTBI uptake. When studying non-transferrin-bound iron (NTBI) uptake by mammalian cells in the high-affinity range (micromolar and below), employment of strong chelators such as NTA can lead to kinetic artifacts due to inhibition by excess chelator. This can be avoided by using citrate at an excess concentration above as a little as1,5-fold over iron without danger of precipitation if balanced culture media are used as incubation mediumfor NTBI uptake. (en)
Title	Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake. Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake. (en)
skos:prefLabel	Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake. Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake. (en)
skos:notation	RIV/68378050:_____/98:23000086!RIV/2003/AV0/A23003/N
http://linked.open.../vavai/riv/strany	128;130
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z5052915)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Kovář, Jan Kriegerbecková, Karin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	778497
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/98:23000086
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	N/A (en)
http://linked.open.../riv/klicoveSlovo	N/A
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E57AB0549305]
http://linked.open...i/riv/nazevZdroje	Analytical Biochemistry
http://linked.open...in/vavai/riv/obor	EB
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http://linked.open...UplatneniVysledku	1998
http://linked.open...v/svazekPeriodika	261
http://linked.open...iv/tvurceVysledku	Kovář, Jan Kriegerbecková, Karin Goldenberg, H. Brunner-Dopper, L.
http://linked.open...n/vavai/riv/zamer	Regulace a signální cesty uplatňující se v genové expresi, imunitě, onkogenezi, replikaci virů, tvorbě buněčných struktur, chování buněk, vývoji a oplození
issn	0003-2697
number of pages	3 (xsd:int)

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