About: Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Gene targeting in mice mainly employs homologous recombination (HR) in embryonic stem (ES) cells. Although it is a standard way for production of genetically modified mice, the procedure is laborious and time-consuming. This study describes targeting of the mouse Rosa26 locus by transcription activator-like effector nucleases (TALENs). We employed TALEN-assisted HR in zygotes to introduce constructs encoding TurboRFP and TagBFP fluorescent proteins into the first intron of the Rosa26 gene, and in this way generated two transgenic mice. We also demonstrated that these Rosa26-specific TALENs exhibit high targeting efficiency superior to that of zinc-finger nucleases (ZFNs) specific for the same targeting sequence. Moreover, we devised a reporter assay to assess TALENs activity and specificity to improve the quality of TALEN-assisted targeting. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. Gene targeting in mice mainly employs homologous recombination (HR) in embryonic stem (ES) cells. Although it is a standard way for production of genetically modified mice, the procedure is laborious and time-consuming. This study describes targeting of the mouse Rosa26 locus by transcription activator-like effector nucleases (TALENs). We employed TALEN-assisted HR in zygotes to introduce constructs encoding TurboRFP and TagBFP fluorescent proteins into the first intron of the Rosa26 gene, and in this way generated two transgenic mice. We also demonstrated that these Rosa26-specific TALENs exhibit high targeting efficiency superior to that of zinc-finger nucleases (ZFNs) specific for the same targeting sequence. Moreover, we devised a reporter assay to assess TALENs activity and specificity to improve the quality of TALEN-assisted targeting. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. (en)
Title	Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases (en)
skos:prefLabel	Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases (en)
skos:notation	RIV/68378050:_____/14:00440940!RIV15-GA0-68378050
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0109), P(GA14-33952S), P(LM2011032)
http://linked.open...iv/cisloPeriodika	21
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hanečková, Radka Sedláček, Radislav Kašpárek, Petr Kříž, Vítězslav Kořínek, Vladimír Krausová, Michaela Žbodáková, Olga
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	13860
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/14:00440940
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Gene targeting; Homologous recombination; Rosa26 locus; Transgenic; Transcription activator-like effector nucleases; Zinc-finger nucleases (en)
http://linked.open.../riv/klicoveSlovo	Rosa26 locus Transcription activator-like effector nucleases Transgenic Zinc-finger nucleases Gene targeting Homologous recombination
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[0D366AE2DA0B]
http://linked.open...i/riv/nazevZdroje	FEBS Letters
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University Infrafrontier-CZ/Czech Centre for Phenogenomics as a national centre of „The European infrastructure for phenotyping and archiving of model mammalian genomes“: Integration of the Czech national centre into international network Molekulární mechanismy regulující diferenciaci střevních kmenových buněk
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	588
http://linked.open...iv/tvurceVysledku	Kříž, Vítězslav Sedláček, Radislav Kořínek, Vladimír Krausová, Michaela Žbodáková, Olga Kašpárek, Petr Hanečková, Radka
http://linked.open...ain/vavai/riv/wos	000344075400029
issn	0014-5793
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.febslet.2014.09.014

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