About: A retrotransposon-driven Dicer isoform directs endogenous small interfering RNA production in mouse oocytes

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About: A retrotransposon-driven Dicer isoform directs endogenous small interfering RNA production in mouse oocytes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In mammals, a single Dicer participates in biogenesis of small RNAs in microRNA (miRNA) and RNAi pathways. In mice, endogenous RNAi is highly active in oocytes, but not in somatic cells, which we ascribe here to an oocyte-specific Dicer isoform (Dicer(O)). Dicer(O) lacks the N-terminal DExD helicase domain and has higher cleavage activity than the full-length Dicer in somatic cells (Dicer(S)). Unlike Dicer(S), Dicer(O) efficiently produces small RNAs from long double-stranded (dsRNA) substrates. Expression of the Dicer(O) isoform is driven by an intronic MT-C retrotransposon promoter, deletion of which causes loss of Dicer(O) and female sterility. Oocytes from females lacking the MT-C element show meiotic spindle defects and increased levels of endogenous small interfering RNA (endo-siRNA) targets, phenocopying the maternal Dicer null phenotype. The alternative Dicer isoform, whose phylogenetic origin demonstrates evolutionary plasticity of RNA-silencing pathways, is the main determinant of endogenous RNAi activity in the mouse female germline. In mammals, a single Dicer participates in biogenesis of small RNAs in microRNA (miRNA) and RNAi pathways. In mice, endogenous RNAi is highly active in oocytes, but not in somatic cells, which we ascribe here to an oocyte-specific Dicer isoform (Dicer(O)). Dicer(O) lacks the N-terminal DExD helicase domain and has higher cleavage activity than the full-length Dicer in somatic cells (Dicer(S)). Unlike Dicer(S), Dicer(O) efficiently produces small RNAs from long double-stranded (dsRNA) substrates. Expression of the Dicer(O) isoform is driven by an intronic MT-C retrotransposon promoter, deletion of which causes loss of Dicer(O) and female sterility. Oocytes from females lacking the MT-C element show meiotic spindle defects and increased levels of endogenous small interfering RNA (endo-siRNA) targets, phenocopying the maternal Dicer null phenotype. The alternative Dicer isoform, whose phylogenetic origin demonstrates evolutionary plasticity of RNA-silencing pathways, is the main determinant of endogenous RNAi activity in the mouse female germline. (en)
Title	A retrotransposon-driven Dicer isoform directs endogenous small interfering RNA production in mouse oocytes A retrotransposon-driven Dicer isoform directs endogenous small interfering RNA production in mouse oocytes (en)
skos:prefLabel	A retrotransposon-driven Dicer isoform directs endogenous small interfering RNA production in mouse oocytes A retrotransposon-driven Dicer isoform directs endogenous small interfering RNA production in mouse oocytes (en)
skos:notation	RIV/68378050:_____/13:00422928!RIV14-GA0-68378050
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0109), P(GA204/09/0085), P(GAP305/10/2215), P(GBP305/12/G034), P(LM2011032)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Sedláček, Radislav Flemr, Matyáš Svoboda, Petr Nejepínská, Jana Malík, Radek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	59009
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/13:00422928
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Dicer; miRNA; RNAi; mouse oocytes (en)
http://linked.open.../riv/klicoveSlovo	mouse oocytes RNAi Dicer miRNA
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B07550E4D3DB]
http://linked.open...i/riv/nazevZdroje	Cell
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University Infrafrontier-CZ/Czech Centre for Phenogenomics as a national centre of „The European infrastructure for phenotyping and archiving of model mammalian genomes“: Integration of the Czech national centre into international network RNA silencing and long dsRNA in mammalian cells Control of chromatin and pluripotency by microRNAs Centre for RNA Biology
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	155
http://linked.open...iv/tvurceVysledku	Sedláček, Radislav Svoboda, Petr Flemr, Matyáš Nejepínská, Jana Malík, Radek Franke, V. Vlahovicek, K.
http://linked.open...ain/vavai/riv/wos	000326934300011
issn	0092-8674
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.cell.2013.10.001

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