About: Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1

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About: Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1 Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The metabotropic glutamate receptor 1 (mGluR1), a class C member of the heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptor family, is a constitutive dimer that regulates excitatory neurotransmission. We investigated the role of homodimer formation in mGluR1 activation by examining activation-dependent inter-and intrasubunit conformational changes by fluorescence resonance energy transfer (FRET). We inserted yellow and cyan fluorescent proteins in the second intracellular loop and at the carboxyl terminus of mGluR1 to act as FRET sensors and expressed these proteins in human embryonic kidney 293 cells. Agonist-dependent activation of these mGluR1 chimeras rapidly increased the intersubunit FRET, suggesting rapid movement of the subunits relative to each other. After intersubunit movement, the intrasubunit FRET decreased, reflecting conformational changes within a subunit. Cotransfection of chimeric receptor subunits that were capable or incapable of G protein coupling revealed that only a single subunit assumes an active state in an mGluR1 receptor dimer. The metabotropic glutamate receptor 1 (mGluR1), a class C member of the heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptor family, is a constitutive dimer that regulates excitatory neurotransmission. We investigated the role of homodimer formation in mGluR1 activation by examining activation-dependent inter-and intrasubunit conformational changes by fluorescence resonance energy transfer (FRET). We inserted yellow and cyan fluorescent proteins in the second intracellular loop and at the carboxyl terminus of mGluR1 to act as FRET sensors and expressed these proteins in human embryonic kidney 293 cells. Agonist-dependent activation of these mGluR1 chimeras rapidly increased the intersubunit FRET, suggesting rapid movement of the subunits relative to each other. After intersubunit movement, the intrasubunit FRET decreased, reflecting conformational changes within a subunit. Cotransfection of chimeric receptor subunits that were capable or incapable of G protein coupling revealed that only a single subunit assumes an active state in an mGluR1 receptor dimer. (en)
Title	Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1 Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1 (en)
skos:prefLabel	Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1 Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1 (en)
skos:notation	RIV/68378050:_____/12:00381622!RIV13-GA0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA303/08/1591), P(GAP303/12/2408), P(LC06063), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	237
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Blahoš, Jaroslav Hlaváčková, Veronika Franková, Daniela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	167530
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/12:00381622
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	G-protein coupled receptor; metabotropic glutamate receptor 1; class C GPCR (en)
http://linked.open.../riv/klicoveSlovo	G-protein coupled receptor class C GPCR metabotropic glutamate receptor 1
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F9265123CCC5]
http://linked.open...i/riv/nazevZdroje	Science Signaling
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Study of glutamate receptors conformational changes using novel fluorescent techniques Functional Consequences of Metabotropic Glutamate Receptor 1a and 1b Splice Variants Assembly in Heterodimeric Complexes
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Blahoš, Jaroslav Batz, J. Franková, Daniela Hlaváčková, Veronika Hoffmann, C. Lohse, M. J. Pin, J. P. Prezeau, L. Zabel, U.
http://linked.open...ain/vavai/riv/wos	000307885200002
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	1937-9145
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1126/scisignal.2002720

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