About: Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53

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About: Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53 Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Immunolocalization and siRNAi cell fractionation plus expression of FLAG-tagged constructs showed presence of γ-tubulin in nucleoli of diverse mammalian interphase cells. It was found outside fibrillar centers of ribosomal DNA transcription, associated with nucleolar remnants after nuclear envelope breakdown and translocated to nucleoli during mitosis. Leptomycin B cell pretreatment did not affect nuclear γ-tubulin distribution, making unlikely rapid active transport of γ-tubulin via nuclear pores into the nucleus. This was confirmed by heterokaryon assay and time-lapse imaging of photoconvertible protein Dendra2 tagged to γ-tubulin. Further experiments revealed and confirmed association of γ-tubulin with tumor suppressor protein C53 located in many subcellular compartments including nucleoli. γ-Tubulin overexpression overcame C53 block of DNA damage G(2)/M checkpoint activation. Beside its known role in microtubule nucleation γ-tubulin may thus also have nuclear-specific function(s). Immunolocalization and siRNAi cell fractionation plus expression of FLAG-tagged constructs showed presence of γ-tubulin in nucleoli of diverse mammalian interphase cells. It was found outside fibrillar centers of ribosomal DNA transcription, associated with nucleolar remnants after nuclear envelope breakdown and translocated to nucleoli during mitosis. Leptomycin B cell pretreatment did not affect nuclear γ-tubulin distribution, making unlikely rapid active transport of γ-tubulin via nuclear pores into the nucleus. This was confirmed by heterokaryon assay and time-lapse imaging of photoconvertible protein Dendra2 tagged to γ-tubulin. Further experiments revealed and confirmed association of γ-tubulin with tumor suppressor protein C53 located in many subcellular compartments including nucleoli. γ-Tubulin overexpression overcame C53 block of DNA damage G(2)/M checkpoint activation. Beside its known role in microtubule nucleation γ-tubulin may thus also have nuclear-specific function(s). (en)
Title	Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53 Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53 (en)
skos:prefLabel	Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53 Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53 (en)
skos:notation	RIV/68378050:_____/12:00370649!RIV13-GA0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0506), P(GA204/09/1777), P(GAP302/10/1701), P(GD204/09/H084), P(KAN200520701), P(LC545), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Philimonenko, Anatolij Hozák, Pavel Vinopal, Stanislav Dráber, Pavel Sládková, Vladimíra Dráberová, Eduarda Sulimenko, Tetyana Sulimenko, Vadym Hořejší, Barbora Vosecká, Věra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	154934
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/12:00370649
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nucleolus; gamma-tubulin; C53 (en)
http://linked.open.../riv/klicoveSlovo	nucleolus C53 gamma-tubulin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[588AFFE5A3DC]
http://linked.open...i/riv/nazevZdroje	Journal of Cellular Physiology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional Organisation of the Cell Center of Molecular and Cellular Immunology Molecular biology of a differentiating cell Nano-PCR - ultrasensitive test for detection of specific proteins in body fluids Analysis of signaling assemblies containing gamma-tubulin Analysis of functional differences between gamma-tubulins
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	227
http://linked.open...iv/tvurceVysledku	Hozák, Pavel Sládková, Vladimíra Dráber, Pavel Dráberová, Eduarda Katsetos, C. D. Philimonenko, Anatoly Sulimenko, Tetyana Vinopal, Stanislav Sulimenko, Vadym Hořejší, Barbora Vosecká, Věra
http://linked.open...ain/vavai/riv/wos	000297942500039
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0021-9541
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jcp.22772

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