About: Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium     Goto   Sponge   NotDistinct   Permalink

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Description
  • Leishmanias are causative agents of leishmaniases and an important biological model for study host-pathogen interaction. Many methods of their cultivation on biphasic and liquid media are available. Biphasic media are superior for initial isolation of parasites and obtaining high promastigote infectivity; liquid media are more suitable for large-scale experiments. Our study adapted and optimized cultivation of Leishmania promastigotes on biphasic SNB-9 (saline–neopeptone–blood 9) originally developed for Trypanosoma cultivation, combining advantages of biphasic and liquid media. We demonstrate that SNB-9 considerably surpasses Schneider's Insect Medium in in vitro infectivity of the parasites and show that the ratio of apoptotic parasites important for the infectivity of the inoculum is higher in SNB-9 culture than in Schneider's Insect Medium culture. Cultivation of Leishmania on SNB-9 thus reliably yields highly infective promastigotes suitable for experimental infection.
  • Leishmanias are causative agents of leishmaniases and an important biological model for study host-pathogen interaction. Many methods of their cultivation on biphasic and liquid media are available. Biphasic media are superior for initial isolation of parasites and obtaining high promastigote infectivity; liquid media are more suitable for large-scale experiments. Our study adapted and optimized cultivation of Leishmania promastigotes on biphasic SNB-9 (saline–neopeptone–blood 9) originally developed for Trypanosoma cultivation, combining advantages of biphasic and liquid media. We demonstrate that SNB-9 considerably surpasses Schneider's Insect Medium in in vitro infectivity of the parasites and show that the ratio of apoptotic parasites important for the infectivity of the inoculum is higher in SNB-9 culture than in Schneider's Insect Medium culture. Cultivation of Leishmania on SNB-9 thus reliably yields highly infective promastigotes suitable for experimental infection. (en)
Title
  • Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium
  • Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium (en)
skos:prefLabel
  • Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium
  • Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium (en)
skos:notation
  • RIV/68378050:_____/11:00371215!RIV12-AV0-68378050
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA310/08/1697), P(LC06009), S, Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 223079
http://linked.open...ai/riv/idVysledku
  • RIV/68378050:_____/11:00371215
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Leishmania; SNB-9; biphasic culture medium; parasite infectivity (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [AE88BA1E8726]
http://linked.open...i/riv/nazevZdroje
  • Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 87
http://linked.open...iv/tvurceVysledku
  • Lipoldová, Marie
  • Svobodová, M.
  • Grekov, Igor
  • Nohýnková, E.
http://linked.open...ain/vavai/riv/wos
  • 000297892200003
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-7012
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.mimet.2011.08.012
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