About: Generation of two modified mouse alleles of the Hic1 tumor suppressor gene

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine %22reporter'' mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence. HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine %22reporter'' mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence. (en)
Title	Generation of two modified mouse alleles of the Hic1 tumor suppressor gene Generation of two modified mouse alleles of the Hic1 tumor suppressor gene (en)
skos:prefLabel	Generation of two modified mouse alleles of the Hic1 tumor suppressor gene Generation of two modified mouse alleles of the Hic1 tumor suppressor gene (en)
skos:notation	RIV/68378050:_____/11:00365113!RIV12-AV0-68378050
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA204/07/1567), P(GD204/09/H058), S, Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Plachý, Jiří Pospíchalová, Vendula Lukáš, Jan Fafílek, Bohumil Vojtěchová, Martina Kořínek, Vladimír Krausová, Michaela Šloncová, Eva Turečková, Jolana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	200945
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/11:00365113
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Hypermethylated In Cancer 1; Hic1 tumor suppressor; gene targeting (en)
http://linked.open.../riv/klicoveSlovo	Hic1 tumor suppressor Hypermethylated In Cancer 1 gene targeting
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D3D3D18E900D]
http://linked.open...i/riv/nazevZdroje	Genesis
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Tumor suppressors in the Wnt signaling pathway Intercellular signalling in development and disease
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	49
http://linked.open...iv/tvurceVysledku	Plachý, Jiří Divoký, V. Fafílek, Bohumil Kořínek, Vladimír Krausová, Michaela Leprince, D. Lukáš, Jan Pospíchalová, Vendula Takacova, S. Turečková, Jolana Vojtěchová, Martina Šloncová, Eva
http://linked.open...ain/vavai/riv/wos	000288815000005
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	1526-954X
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/dvg.20719

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