About: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Quantitative real-time PCR (qPCR) is becoming increasingly important for DNA genotyping and gene expression analysis. Buffers of different composition are used for various DNA dyes used in qPCR. In search for a PCR mix compatible with variety of DNA dyes, and suitable for efficient amplification of difficult-to-amplify DNA templates, such as those in whole blood, of medium size and/or GC-rich, we found excellent performance of a PCR mix supplemented with 1 M 1,2-propanediol and 0.2 M trehalose (PT enhancer). These two additives together decreased DNA melting temperature and efficiently neutralized PCR inhibitors present in blood samples. They also made possible more efficient amplification of GC-rich templates than betaine and other previously described additives. The data indicate that PCR mixes supplemented with PT enhancer are suitable for DNA amplification in the presence of various DNA dyes and for a variety of templates which otherwise can be amplified with difficulty. Quantitative real-time PCR (qPCR) is becoming increasingly important for DNA genotyping and gene expression analysis. Buffers of different composition are used for various DNA dyes used in qPCR. In search for a PCR mix compatible with variety of DNA dyes, and suitable for efficient amplification of difficult-to-amplify DNA templates, such as those in whole blood, of medium size and/or GC-rich, we found excellent performance of a PCR mix supplemented with 1 M 1,2-propanediol and 0.2 M trehalose (PT enhancer). These two additives together decreased DNA melting temperature and efficiently neutralized PCR inhibitors present in blood samples. They also made possible more efficient amplification of GC-rich templates than betaine and other previously described additives. The data indicate that PCR mixes supplemented with PT enhancer are suitable for DNA amplification in the presence of various DNA dyes and for a variety of templates which otherwise can be amplified with difficulty. (en)
Title	1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer (en)
skos:prefLabel	1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer (en)
skos:notation	RIV/68378050:_____/11:00365065!RIV12-AV0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/09/1826), P(GAP302/10/1759), P(GD204/09/H084), P(KAN200520701), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Dráber, Petr Dráberová, Lubica Bugajev, Viktor Horáková, Helena Eitler, Jiří Polakovičová, Iva Shaik, Gouse Mohiddin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	243921
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/11:00365065
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	qPCR enhancer; trehalose; 1,2-propanediol (en)
http://linked.open.../riv/klicoveSlovo	1 2-propanediol qPCR enhancer trehalose
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[0E6738BFFFA5]
http://linked.open...i/riv/nazevZdroje	BMC Biotechnology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular biology of a differentiating cell Nano-PCR - ultrasensitive test for detection of specific proteins in body fluids Topography and function of Csk-binding proteins of the plasma membrane in mast cells Function-structure relationships between transmembrane adaptor-based signalosomes in mast cells
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Dráber, Petr Bugajev, Viktor Dráberová, Lubica Eitler, Jiří Horáková, Helena Polakovičová, Iva Shaik, Gouse Mohiddin
http://linked.open...ain/vavai/riv/wos	000290988500001
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	1472-6750
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1186/1472-6750-11-41

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