About: Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling

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About: Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mammalian Wnt ligands contain two types of post-translational modification: the covalent attachment of fatty acids at two distinct positions, and the N-glycosylation of multiple asparagines. We examined how these modifications contribute to the secretion, extracellular movement and signaling activity of mouse Wnt1 and Wnt3a ligands. We revealed that O-linked acylation of serine is required for the subsequent S-palmitoylation of cysteine. Interestingly, although double-acylation of Wnt1 was indispensable for signaling in mammalian cells, in Xenopus embryos the S-palmitoyl-deficient form retained the signaling activity. In the case of Wnt3a, the functional duality of the attached acyls was less prominent, since the ligand lacking S-linked palmitate was still capable of signaling in various cellular contexts. Finally, we show that the signaling competency of both Wnt1 and Wnt3a is related to their ability to associate with the extracellular matrix. Mammalian Wnt ligands contain two types of post-translational modification: the covalent attachment of fatty acids at two distinct positions, and the N-glycosylation of multiple asparagines. We examined how these modifications contribute to the secretion, extracellular movement and signaling activity of mouse Wnt1 and Wnt3a ligands. We revealed that O-linked acylation of serine is required for the subsequent S-palmitoylation of cysteine. Interestingly, although double-acylation of Wnt1 was indispensable for signaling in mammalian cells, in Xenopus embryos the S-palmitoyl-deficient form retained the signaling activity. In the case of Wnt3a, the functional duality of the attached acyls was less prominent, since the ligand lacking S-linked palmitate was still capable of signaling in various cellular contexts. Finally, we show that the signaling competency of both Wnt1 and Wnt3a is related to their ability to associate with the extracellular matrix. (en)
Title	Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling (en)
skos:prefLabel	Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling (en)
skos:notation	RIV/68378050:_____/11:00364988!RIV12-AV0-68378050
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(1M0506), P(GA204/07/1567), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Tůmová, Lucie Plachý, Jiří Pospíchalová, Vendula Lukáš, Jan Doubravská, Lenka Valenta, Tomáš Fafílek, Bohumil Vojtěchová, Martina Kořínek, Vladimír Krausová, Michaela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	199416
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/11:00364988
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Wnt signaling; post-translational modification; acylation (en)
http://linked.open.../riv/klicoveSlovo	Wnt signaling acylation post-translational modification
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[9E345ADB97EB]
http://linked.open...i/riv/nazevZdroje	Cellular Signalling
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Tumor suppressors in the Wnt signaling pathway Center of Molecular and Cellular Immunology
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Plachý, Jiří Tůmová, Lucie Valenta, Tomáš Doubravská, Lenka Fafílek, Bohumil Gradl, D. Kořínek, Vladimír Krausová, Michaela Lukáš, Jan Pospíchalová, Vendula Vojtěchová, Martina Šebesta, O.
http://linked.open...ain/vavai/riv/wos	000288977400011
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0898-6568
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.cellsig.2011.01.007

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