About: Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics

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About: Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We prepared peptides containing four repeats of the sequences VAALEKE (peptide E) or VAALKEK (peptide K). While the peptides alone adopt in aqueous solutions a random coil conformation, their equimolar mixture forms heterodimeric coiled coils as confirmed by CD spectroscopy. A terminal azide group enabled conjugation of the peptide with a synthetic drug carrier based on the N-(2-hydroxypropyl)methacrylamide copolymer containing propargyl groups using “click chemistry. When incorporated into the polymer drug carrier, peptide E formed a stable noncovalent complex with peptide K belonging to a recombinant single-chain fragment (scFv) of the M75 antibody. The complex mediated a noncovalent linkage between the polymer drug carrier and the protein. The antigen binding affinity of the polymerscFv complex was confirmed by ELISA. This approach offers a well-defined, specific, and nondestructive universal method for the preparation of protein-targeted polymer drug carriers. We prepared peptides containing four repeats of the sequences VAALEKE (peptide E) or VAALKEK (peptide K). While the peptides alone adopt in aqueous solutions a random coil conformation, their equimolar mixture forms heterodimeric coiled coils as confirmed by CD spectroscopy. A terminal azide group enabled conjugation of the peptide with a synthetic drug carrier based on the N-(2-hydroxypropyl)methacrylamide copolymer containing propargyl groups using “click chemistry. When incorporated into the polymer drug carrier, peptide E formed a stable noncovalent complex with peptide K belonging to a recombinant single-chain fragment (scFv) of the M75 antibody. The complex mediated a noncovalent linkage between the polymer drug carrier and the protein. The antigen binding affinity of the polymerscFv complex was confirmed by ELISA. This approach offers a well-defined, specific, and nondestructive universal method for the preparation of protein-targeted polymer drug carriers. (en)
Title	Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics (en)
skos:prefLabel	Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics (en)
skos:notation	RIV/68378050:_____/11:00364976!RIV12-AV0-68378050
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(1M0505), P(GA203/08/0543), Z(AV0Z40500505), Z(AV0Z40550506), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Král, Vlastimil Fábry, Milan Sieglová, Irena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	190801
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/11:00364976
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	coiled coil; polymer therapeutics; drug targeting (en)
http://linked.open.../riv/klicoveSlovo	coiled coil drug targeting polymer therapeutics
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[6EE773254AC1]
http://linked.open...i/riv/nazevZdroje	Biomacromolecules
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs Hybrid macromolecular systems based on hydrophilic polymers and stimuli-responsive peptides
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Bednárová, Lucie Fábry, Milan Král, Vlastimil Laga, Richard Maloň, Petr Pechar, Michal Pola, Robert Sieglová, Irena Ulbrich, Karel Vaněk, O.
http://linked.open...ain/vavai/riv/wos	000295602600032
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	1525-7797
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/bm200897b

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