About: Enzymatic synthesis of oligo-D-galactofuranosides and l-arabinofuranosides: from molecular dynamics to immunological assays

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	D-Galactofuranosyl-containing conjugates are ubiquitous in many pathogenic microorganisms, but completely absent from mammals. Based on the similarity between chemical structures, the arabinofuranosyl hydrolase Araf51 from Clostridium thermocellum was not only able to hydrolyze galactosyl derivatives, but was also really efficient in catalyzing oligomerisations using p-nitrophenyl furanosides as donors. The structures of the products obtained were determined using MS and NMR approaches. Amongst them, all the possible regioisomers of di-arabino and -galactofuranosides were synthesized. The galactofuranobioside displaying the biologically relevant sequence beta-D-Galf-(1,6)-beta-D-Galf was enzymatically prepared for the first time. All fractions going from di- to penta-arabino- and galactofuranosides were tested for their ability in eliciting the production of TNF-alpha. Interesting immunological properties were observed with arabinofuranosides as short as three sugar residues. D-Galactofuranosyl-containing conjugates are ubiquitous in many pathogenic microorganisms, but completely absent from mammals. Based on the similarity between chemical structures, the arabinofuranosyl hydrolase Araf51 from Clostridium thermocellum was not only able to hydrolyze galactosyl derivatives, but was also really efficient in catalyzing oligomerisations using p-nitrophenyl furanosides as donors. The structures of the products obtained were determined using MS and NMR approaches. Amongst them, all the possible regioisomers of di-arabino and -galactofuranosides were synthesized. The galactofuranobioside displaying the biologically relevant sequence beta-D-Galf-(1,6)-beta-D-Galf was enzymatically prepared for the first time. All fractions going from di- to penta-arabino- and galactofuranosides were tested for their ability in eliciting the production of TNF-alpha. Interesting immunological properties were observed with arabinofuranosides as short as three sugar residues. (en)
Title	Enzymatic synthesis of oligo-D-galactofuranosides and l-arabinofuranosides: from molecular dynamics to immunological assays Enzymatic synthesis of oligo-D-galactofuranosides and l-arabinofuranosides: from molecular dynamics to immunological assays (en)
skos:prefLabel	Enzymatic synthesis of oligo-D-galactofuranosides and l-arabinofuranosides: from molecular dynamics to immunological assays Enzymatic synthesis of oligo-D-galactofuranosides and l-arabinofuranosides: from molecular dynamics to immunological assays (en)
skos:notation	RIV/68378050:_____/10:00349850!RIV11-AV0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(MEB020824), Z(AV0Z50520514), Z(MSM6046137305)
http://linked.open...iv/cisloPeriodika	8-9
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Filipp, Dominik
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	257349
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/10:00349850
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	enzymatic synthesis; oligofuranosides; immune stimulation (en)
http://linked.open.../riv/klicoveSlovo	enzymatic synthesis immune stimulation oligofuranosides
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[6DFFFEAA8258]
http://linked.open...i/riv/nazevZdroje	Organic & Biomolecular Chemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	New glycosynthases and their use in glycoconjugates synthesis
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Dvořáková, H. Spiwok, V. Filipp, Dominik Králová, B. Chlubnová, I. Daniellou, R. Ferrieres, V. Nugier-Chauvin, C.
http://linked.open...ain/vavai/riv/wos	000276786000016
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů Teoretické základy potravinářských a biochemických technologií
issn	1477-0520
number of pages	11 (xsd:int)

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